Immunization of rhesus macaques with a polyvalent DNA prime/protein boost human immunodeficiency virus type 1 vaccine elicits protective antibody response against simian human immunodeficiency virus of R5 phenotype.

Published

Journal Article

The immunogenicity of a poylvalent HIV-1 vaccine comprised of Env antigens from primary R5 isolates was evaluated in rhesus macaques. DNA vaccines encoding four Env antigens from multiple HIV-1 subtypes and HIV-1 Gag antigen from a single subtype elicited a persistent level of binding antibodies to gp120 from multiple HIV-1 isolates that were markedly enhanced following boosting with homologous gp120 proteins in QS-21 adjuvant irrespective of the route of DNA immunization. These sera neutralized homologous and, to a lesser degree, heterologous HIV-1 isolates. Four of the six immunized animals were completely protected following rectal challenge with a SHIV encoding Env from HIV-1(Ba-L), whereas the virus load was reduced in the remaining animals compared to naïve controls. Hence priming with DNA encoding Env antigens from multiple HIV-1 clades followed by boosting with homologous Env proteins elicits anti-HIV-1 immune responses capable of protecting macaques against mucosal transmission of R5 tropic SHIV isolate.

Full Text

Duke Authors

Cited Authors

  • Pal, R; Kalyanaraman, VS; Nair, BC; Whitney, S; Keen, T; Hocker, L; Hudacik, L; Rose, N; Mboudjeka, I; Shen, S; Wu-Chou, T-H; Montefiori, D; Mascola, J; Markham, P; Lu, S

Published Date

  • May 10, 2006

Published In

Volume / Issue

  • 348 / 2

Start / End Page

  • 341 - 353

PubMed ID

  • 16460776

Pubmed Central ID

  • 16460776

International Standard Serial Number (ISSN)

  • 0042-6822

Digital Object Identifier (DOI)

  • 10.1016/j.virol.2005.12.029

Language

  • eng

Conference Location

  • United States