Vaccine evaluation studies of replication-defective SIVsmB7.

Published

Journal Article

Non-infectious virus-like particles of SIVsmB7 that expresses env and gag gene products but are defective in pol and vpx/vpr were assessed for their ability to induce protective immunity against infection with pathogenic SIVsmE660 in rhesus macaques. Animals were immunized in three groups: group A was primed with cell-associated SIVsmB7 and boosted with cell-free SIVsmB7; group B was primed with cell-free SIVsmB7 and boosted with cell-free SIVsmB7 conjugated to iron oxide microbeads; group C was primed with cell-free SIVsmB7 mixed with Titer Max adjuvant and boosted with cell-free SIVsmB7 mixed with SAF-M adjuvant followed by secondary boosting with cell-free SIVsmB7 conjugated to microbeads. Animals were challenged intravenously with 20 animal infectious doses of SIVsmE660 grown in rhesus peripheral blood mononuclear cells 3 weeks after final boosting. All animals became infected as evidenced by quantitative virus cultivation. Sera from immunized animals contained low-titer antibodies by ELISA and low or undetectable neutralizing antibodies on the day of challenge but strong anamnestic antibody responses were observed following challenge. Interestingly, 2 of 3 animals in group A showed evidence of transient viremia and more stable CD4 counts following challenge as compared to the other immunized animals and to non-immunized controls. Thus, immunization with cell-associated SIVsmB7 did not provide sterilizing immunity against challenge with a highly pathogenic SIV strain but might have caused virus clearance later in infection.

Full Text

Duke Authors

Cited Authors

  • Kraiselburd, EN; Salaman, A; Beltrán, M; Rivera, M; Oliver, J; Kessler, M; Knezevich, M; Rodriguez, A; Bilska, M; Montefiori, D; Torres-Bauza, LJ; Martinez, I

Published Date

  • November 1997

Published In

Volume / Issue

  • 43 / 7

Start / End Page

  • 915 - 924

PubMed ID

  • 9449524

Pubmed Central ID

  • 9449524

International Standard Serial Number (ISSN)

  • 0145-5680

Language

  • eng

Conference Location

  • France