Potent, protective anti-HIV immune responses generated by bimodal HIV envelope DNA plus protein vaccination.

Published

Journal Article

It is generally thought that an effective vaccine to prevent HIV-1 infection should elicit both strong neutralizing antibody and cytotoxic T lymphocyte responses. We recently demonstrated that potent, boostable, long-lived HIV-1 envelope (Env)-specific cytotoxic T lymphocyte responses can be elicited in rhesus monkeys using plasmid-encoded HIV-1 env DNA as the immunogen. In the present study, we show that the addition of HIV-1 Env protein to this regimen as a boosting immunogen generates a high titer neutralizing antibody response in this nonhuman primate species. Moreover, we demonstrate in a pilot study that immunization with HIV-1 env DNA (multiple doses) followed by a final immunization with HIV-1 env DNA plus HIV-1 Env protein (env gene from HXBc2 clone of HIV IIIB; Env protein from parental HIV IIIB) completely protects monkeys from infection after i.v. challenge with a chimeric virus expressing HIV-1 env (HXBc2) on a simian immmunodeficiency virusmac backbone (SHIV-HXBc2). The potent immunity and protection seen in these pilot experiments suggest that a DNA prime/DNA plus protein boost regimen warrants active investigation as a vaccine strategy to prevent HIV-1 infection.

Full Text

Duke Authors

Cited Authors

  • Letvin, NL; Montefiori, DC; Yasutomi, Y; Perry, HC; Davies, ME; Lekutis, C; Alroy, M; Freed, DC; Lord, CI; Handt, LK; Liu, MA; Shiver, JW

Published Date

  • August 19, 1997

Published In

Volume / Issue

  • 94 / 17

Start / End Page

  • 9378 - 9383

PubMed ID

  • 9256490

Pubmed Central ID

  • 9256490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.94.17.9378

Language

  • eng

Conference Location

  • United States