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Studies of the conformation-dependent neutralizing epitopes of simian immunodeficiency virus envelope protein.

Publication ,  Journal Article
Javaherian, K; Langlois, AJ; Montefiori, DC; Kent, KA; Ryan, KA; Wyman, PD; Stott, J; Bolognesi, DP; Murphey-Corb, M; Larosa, GJ
Published in: J Virol
April 1994

It has been shown previously that the major neutralizing epitopes in simian immunodeficiency virus (SIV) are discontinuous and conformation dependent and that the V3 loop, in contrast to that of human immunodeficiency virus (HIV) type 1, does not by itself elicit neutralizing antibodies (K. Javaherian et al., Proc. Natl. Acad. Sci. USA 89:1418-1422, 1992). We now present data showing that on the basis of fractionation of infected macaque sera, protease digestion of the envelope, and binding properties of two neutralizing monoclonal antibodies to SIV and SIV-HIV chimeric envelope proteins, changes in V3 can disrupt the conformation-dependent neutralization region. The chimeric protein did not produce significant neutralizing antibodies against either SIV or HIV. We also report that neutralizing antibodies elicited by recombinant SIV envelope proteins of mac251 and B670 isolates cross-neutralize. Finally, we show that deglycosylation of the SIV envelope results in a molecule which binds neither soluble CD4 nor the neutralizing monoclonal antibodies being investigated here and does not elicit sera with a significant neutralizing titer.

Duke Scholars

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

April 1994

Volume

68

Issue

4

Start / End Page

2624 / 2631

Location

United States

Related Subject Headings

  • Virology
  • Viral Envelope Proteins
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Retroviridae Proteins
  • Recombinant Fusion Proteins
  • Protein Processing, Post-Translational
  • Protein Conformation
  • Protein Binding
  • Peptide Fragments
 

Citation

APA
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ICMJE
MLA
NLM
Javaherian, K., Langlois, A. J., Montefiori, D. C., Kent, K. A., Ryan, K. A., Wyman, P. D., … Larosa, G. J. (1994). Studies of the conformation-dependent neutralizing epitopes of simian immunodeficiency virus envelope protein. J Virol, 68(4), 2624–2631. https://doi.org/10.1128/JVI.68.4.2624-2631.1994
Javaherian, K., A. J. Langlois, D. C. Montefiori, K. A. Kent, K. A. Ryan, P. D. Wyman, J. Stott, D. P. Bolognesi, M. Murphey-Corb, and G. J. Larosa. “Studies of the conformation-dependent neutralizing epitopes of simian immunodeficiency virus envelope protein.J Virol 68, no. 4 (April 1994): 2624–31. https://doi.org/10.1128/JVI.68.4.2624-2631.1994.
Javaherian K, Langlois AJ, Montefiori DC, Kent KA, Ryan KA, Wyman PD, et al. Studies of the conformation-dependent neutralizing epitopes of simian immunodeficiency virus envelope protein. J Virol. 1994 Apr;68(4):2624–31.
Javaherian, K., et al. “Studies of the conformation-dependent neutralizing epitopes of simian immunodeficiency virus envelope protein.J Virol, vol. 68, no. 4, Apr. 1994, pp. 2624–31. Pubmed, doi:10.1128/JVI.68.4.2624-2631.1994.
Javaherian K, Langlois AJ, Montefiori DC, Kent KA, Ryan KA, Wyman PD, Stott J, Bolognesi DP, Murphey-Corb M, Larosa GJ. Studies of the conformation-dependent neutralizing epitopes of simian immunodeficiency virus envelope protein. J Virol. 1994 Apr;68(4):2624–2631.

Published In

J Virol

DOI

ISSN

0022-538X

Publication Date

April 1994

Volume

68

Issue

4

Start / End Page

2624 / 2631

Location

United States

Related Subject Headings

  • Virology
  • Viral Envelope Proteins
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Retroviridae Proteins
  • Recombinant Fusion Proteins
  • Protein Processing, Post-Translational
  • Protein Conformation
  • Protein Binding
  • Peptide Fragments