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CD4-independent binding of HIV-1 to the B lymphocyte receptor CR2 (CD21) in the presence of complement and antibody.

Publication ,  Journal Article
Montefiori, DC; Zhou, J; Shaff, DI
Published in: Clin Exp Immunol
December 1992

Complement and antibody contribute to infection-enhancement and possible expanded cellular tropism of HIV-1 in vitro through a process requiring complement receptors. Until now, however, the ability of HIV-1 to bind complement receptors has not been documented or characterized. We investigated whether antibody and complement permitted HIV-1 to bind to the B lymphocyte receptor, CR2 (CD21), in an effort to learn more about infection-enhancement, and also because CR2 can mediate B cell proliferation and antigen localization in lymphoid organs in other systems. HIV-1 incubated with antibody and fresh human serum as a source of complement bound approximately 10-fold greater to cells expressing CR2 than to HIV-1-permissive cells lacking this receptor. A similar effect was observed using cells which expressed CR2 but no CD4. This binding was minimal in heat-inactivated and C3-deficient sera, and was significantly reduced by the anti-CR2 MoAb, OKB7, but not by the anti-CD4 MoAb, OKT4a. Thus, complement and antibody acted in concert to facilitate the binding of HIV-1 to CR2 independently of CD4. CD4-independent binding of HIV-1 to CR2 was not sufficient to produce infection in Raji-3 cells. Titres of antibodies mediating CR2 binding correlated with antibody titres as measured by immunofluorescence (P < 0.01) and infection-enhancement (P < 0.05) but were discordant with titres of neutralizing antibodies, a result consistent with the utilization of CR2 for enhanced infection of cells. The ability of complement and antibody to facilitate the binding of HIV-1 to CR2 in the absence of CD4 provides new insights into mechanisms of HIV-1-induced immunopathogenesis and infection-enhancement.

Duke Scholars

Published In

Clin Exp Immunol

DOI

ISSN

0009-9104

Publication Date

December 1992

Volume

90

Issue

3

Start / End Page

383 / 389

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Receptors, Virus
  • Receptors, Immunologic
  • Receptors, HIV
  • Immunology
  • Humans
  • HIV-1
  • Complement System Proteins
  • CD2 Antigens
  • Burkitt Lymphoma
 

Citation

APA
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ICMJE
MLA
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Montefiori, D. C., Zhou, J., & Shaff, D. I. (1992). CD4-independent binding of HIV-1 to the B lymphocyte receptor CR2 (CD21) in the presence of complement and antibody. Clin Exp Immunol, 90(3), 383–389. https://doi.org/10.1111/j.1365-2249.1992.tb05855.x
Montefiori, D. C., J. Zhou, and D. I. Shaff. “CD4-independent binding of HIV-1 to the B lymphocyte receptor CR2 (CD21) in the presence of complement and antibody.Clin Exp Immunol 90, no. 3 (December 1992): 383–89. https://doi.org/10.1111/j.1365-2249.1992.tb05855.x.
Montefiori, D. C., et al. “CD4-independent binding of HIV-1 to the B lymphocyte receptor CR2 (CD21) in the presence of complement and antibody.Clin Exp Immunol, vol. 90, no. 3, Dec. 1992, pp. 383–89. Pubmed, doi:10.1111/j.1365-2249.1992.tb05855.x.
Journal cover image

Published In

Clin Exp Immunol

DOI

ISSN

0009-9104

Publication Date

December 1992

Volume

90

Issue

3

Start / End Page

383 / 389

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Receptors, Virus
  • Receptors, Immunologic
  • Receptors, HIV
  • Immunology
  • Humans
  • HIV-1
  • Complement System Proteins
  • CD2 Antigens
  • Burkitt Lymphoma