Complement-mediated antibody-dependent enhancement of HIV-1 infection requires CD4 and complement receptors.
Journal Article (Journal Article)
In this study it is demonstrated that complement-mediated antibody-dependent enhancement (C'-ADE) of HIV-1 infection in vitro is blocked by murine monoclonal antibodies to CD4 and complement receptor type 2 (CR2) while HIV-1 infection in the absence of C'-ADE is blocked by anti-CD4 but not anti-CR2 monoclonal antibodies. The anti-CR2 murine monoclonal antibody, OKB7, blocked C'-ADE of HIV-1 infection at concentrations greater than 1 microgram/ml. The anti-CD4 monoclonal antibody, OKT4a, but not OKT4f blocked C'-ADE at concentrations greater than 0.06 microgram/ml. HIV-1 infections were quantitated by cytopathic effect, indirect immunofluorescence, and reverse transcriptase release. It appears from these in vitro studies that C'-ADE of HIV-1 infection requires both CD4 and complement receptors while HIV-1 infection in the absence of antibody and complement requires only CD4.
Full Text
Duke Authors
Cited Authors
- Robinson, WE; Montefiori, DC; Mitchell, WM
Published Date
- April 1990
Published In
Volume / Issue
- 175 / 2
Start / End Page
- 600 - 604
PubMed ID
- 2327077
International Standard Serial Number (ISSN)
- 0042-6822
Digital Object Identifier (DOI)
- 10.1016/0042-6822(90)90449-2
Language
- eng
Conference Location
- United States