Complement-mediated antibody-dependent enhancement of HIV-1 infection requires CD4 and complement receptors.
In this study it is demonstrated that complement-mediated antibody-dependent enhancement (C'-ADE) of HIV-1 infection in vitro is blocked by murine monoclonal antibodies to CD4 and complement receptor type 2 (CR2) while HIV-1 infection in the absence of C'-ADE is blocked by anti-CD4 but not anti-CR2 monoclonal antibodies. The anti-CR2 murine monoclonal antibody, OKB7, blocked C'-ADE of HIV-1 infection at concentrations greater than 1 microgram/ml. The anti-CD4 monoclonal antibody, OKT4a, but not OKT4f blocked C'-ADE at concentrations greater than 0.06 microgram/ml. HIV-1 infections were quantitated by cytopathic effect, indirect immunofluorescence, and reverse transcriptase release. It appears from these in vitro studies that C'-ADE of HIV-1 infection requires both CD4 and complement receptors while HIV-1 infection in the absence of antibody and complement requires only CD4.
Duke Scholars
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- Virology
- Receptors, Complement
- Humans
- HIV-1
- HIV Antibodies
- Flow Cytometry
- Cytopathogenic Effect, Viral
- Complement System Proteins
- Cell Line
- CD4 Antigens
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Virology
- Receptors, Complement
- Humans
- HIV-1
- HIV Antibodies
- Flow Cytometry
- Cytopathogenic Effect, Viral
- Complement System Proteins
- Cell Line
- CD4 Antigens