Complement-mediated antibody-dependent enhancement of HIV-1 infection requires CD4 and complement receptors.

Published

Journal Article

In this study it is demonstrated that complement-mediated antibody-dependent enhancement (C'-ADE) of HIV-1 infection in vitro is blocked by murine monoclonal antibodies to CD4 and complement receptor type 2 (CR2) while HIV-1 infection in the absence of C'-ADE is blocked by anti-CD4 but not anti-CR2 monoclonal antibodies. The anti-CR2 murine monoclonal antibody, OKB7, blocked C'-ADE of HIV-1 infection at concentrations greater than 1 microgram/ml. The anti-CD4 monoclonal antibody, OKT4a, but not OKT4f blocked C'-ADE at concentrations greater than 0.06 microgram/ml. HIV-1 infections were quantitated by cytopathic effect, indirect immunofluorescence, and reverse transcriptase release. It appears from these in vitro studies that C'-ADE of HIV-1 infection requires both CD4 and complement receptors while HIV-1 infection in the absence of antibody and complement requires only CD4.

Full Text

Duke Authors

Cited Authors

  • Robinson, WE; Montefiori, DC; Mitchell, WM

Published Date

  • April 1990

Published In

Volume / Issue

  • 175 / 2

Start / End Page

  • 600 - 604

PubMed ID

  • 2327077

Pubmed Central ID

  • 2327077

International Standard Serial Number (ISSN)

  • 0042-6822

Digital Object Identifier (DOI)

  • 10.1016/0042-6822(90)90449-2

Language

  • eng

Conference Location

  • United States