Differential inhibition of HIV-1 cell binding and HIV-1-induced syncytium formation by low molecular weight sulphated polysaccharides.

Published

Journal Article

Dextran sulphate (MW 5000 and 8000) and a polysulphated glycosaminoglycan (MW 10,000), at concentrations that provided complete protection in a homologous infection assay, failed to block syncytium formation and the resulting cytopathic effect when MT-2 cells were mixed with H9/HIV-1 cells. These substances also had no antiviral activity when added to cells, after virus challenge, at a time when binding and entry were complete. However, a high molecular weight (500,000) dextran sulphate blocked HIV-1 infection at both stages. Thus, the gp120-CD4 interactions mediating HIV-1 binding and HIV-1-induced syncytium formation are differentially affected by this class of polyanionic substances. Furthermore, size may be a determining factor in their potential application as anti-HIV treatment.

Full Text

Duke Authors

Cited Authors

  • Montefiori, DC; Robinson, WE; Modliszewski, A; Rowland, JM; Schuffman, SS; Mitchell, WM

Published Date

  • March 1, 1990

Published In

Volume / Issue

  • 25 / 3

Start / End Page

  • 313 - 318

PubMed ID

  • 1692572

Pubmed Central ID

  • 1692572

International Standard Serial Number (ISSN)

  • 0305-7453

Digital Object Identifier (DOI)

  • 10.1093/jac/25.3.313

Language

  • eng

Conference Location

  • England