Antibody-independent, complement-mediated enhancement of HIV-1 infection by mannosidase I and II inhibitors.

Published

Journal Article

Human immunodeficiency virus type 1 (HIV-1) infectivity and cytopathic effect require proper maturation of the viral envelope glycoprotein carbohydrate moieties. We have found that fresh human serum enhances the infectivity of HIV-1 in MT-2 cell infection assays when virus is synthesized in the presence of the mannosidase I inhibitor, 1-deoxymannojirimycin, or the mannosidase II inhibitor, swainsonine, but has no enhancing effect on virus synthesized in the presence of the glucosidase I inhibitors, castanospermine and 1-deoxynojirimycin, or the glucosidase II inhibitor, bromoconduritol. Enhanced infections were characterized by cytopathic effect, antigen synthesis and reverse transcriptase release, all which occurred sooner than in control-infected cultures. This enhancement of infection was also observed in C1q-deficient serum but was not observed in serum that was heat-inactivated or depleted of complement components C3 or factor B, thus suggesting a requirement for the alternate pathway of complement.

Full Text

Duke Authors

Cited Authors

  • Montefiori, DC; Robinson, WE; Mitchell, WM

Published Date

  • April 1989

Published In

Volume / Issue

  • 11 / 3

Start / End Page

  • 137 - 146

PubMed ID

  • 2472115

Pubmed Central ID

  • 2472115

International Standard Serial Number (ISSN)

  • 0166-3542

Language

  • eng

Conference Location

  • Netherlands