Inhibition of HIV-1 proviral DNA synthesis and RNA accumulation by mismatched dsRNA.

Published

Journal Article

The antiviral activity of mismatched dsRNA of the form poly(I):poly(C12-U)n (Ampligen) against the human immunodeficiency virus type 1 (HIV-1) was investigated by RNA-RNA and RNA-DNA hybridizations. Mismatched dsRNA delayed the appearance of newly transcribed HIV-1 RNA as detected by liquid dot-blot hybridization in cultures of H9 T-lymphoblastoid cells following virus challenge. The appearance of proviral DNA as detected by Southern hybridization following virus challenge in H9 cells was also delayed. Mismatched dsRNA had no effect in syncytium inhibition assays performed by fusing MT-2 cells with H9/HTLV-IIIB cells. These results suggest that the in vitro anti-HIV-1 activity of mismatched dsRNA occurs, at least in part, at an early stage in the viral replication cycle following initial gp120-CD4 binding.

Full Text

Duke Authors

Cited Authors

  • Montefiori, DC; Pellegrino, MG; Robinson, WE; Engle, K; Field, M; Mitchell, WM; Gillespie, DH

Published Date

  • February 15, 1989

Published In

Volume / Issue

  • 158 / 3

Start / End Page

  • 943 - 950

PubMed ID

  • 2784055

Pubmed Central ID

  • 2784055

International Standard Serial Number (ISSN)

  • 0006-291X

Digital Object Identifier (DOI)

  • 10.1016/0006-291x(89)92813-1

Language

  • eng

Conference Location

  • United States