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Inhibition of HIV-1 proviral DNA synthesis and RNA accumulation by mismatched dsRNA.

Publication ,  Journal Article
Montefiori, DC; Pellegrino, MG; Robinson, WE; Engle, K; Field, M; Mitchell, WM; Gillespie, DH
Published in: Biochem Biophys Res Commun
February 15, 1989

The antiviral activity of mismatched dsRNA of the form poly(I):poly(C12-U)n (Ampligen) against the human immunodeficiency virus type 1 (HIV-1) was investigated by RNA-RNA and RNA-DNA hybridizations. Mismatched dsRNA delayed the appearance of newly transcribed HIV-1 RNA as detected by liquid dot-blot hybridization in cultures of H9 T-lymphoblastoid cells following virus challenge. The appearance of proviral DNA as detected by Southern hybridization following virus challenge in H9 cells was also delayed. Mismatched dsRNA had no effect in syncytium inhibition assays performed by fusing MT-2 cells with H9/HTLV-IIIB cells. These results suggest that the in vitro anti-HIV-1 activity of mismatched dsRNA occurs, at least in part, at an early stage in the viral replication cycle following initial gp120-CD4 binding.

Duke Scholars

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

February 15, 1989

Volume

158

Issue

3

Start / End Page

943 / 950

Location

United States

Related Subject Headings

  • Zidovudine
  • Transcription, Genetic
  • T-Lymphocytes
  • Recombinant Proteins
  • RNA, Viral
  • RNA, Double-Stranded
  • Polyribonucleotides
  • Poly U
  • Poly I-C
  • Nucleic Acid Hybridization
 

Citation

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Montefiori, D. C., Pellegrino, M. G., Robinson, W. E., Engle, K., Field, M., Mitchell, W. M., & Gillespie, D. H. (1989). Inhibition of HIV-1 proviral DNA synthesis and RNA accumulation by mismatched dsRNA. Biochem Biophys Res Commun, 158(3), 943–950. https://doi.org/10.1016/0006-291x(89)92813-1
Montefiori, D. C., M. G. Pellegrino, W. E. Robinson, K. Engle, M. Field, W. M. Mitchell, and D. H. Gillespie. “Inhibition of HIV-1 proviral DNA synthesis and RNA accumulation by mismatched dsRNA.Biochem Biophys Res Commun 158, no. 3 (February 15, 1989): 943–50. https://doi.org/10.1016/0006-291x(89)92813-1.
Montefiori DC, Pellegrino MG, Robinson WE, Engle K, Field M, Mitchell WM, et al. Inhibition of HIV-1 proviral DNA synthesis and RNA accumulation by mismatched dsRNA. Biochem Biophys Res Commun. 1989 Feb 15;158(3):943–50.
Montefiori, D. C., et al. “Inhibition of HIV-1 proviral DNA synthesis and RNA accumulation by mismatched dsRNA.Biochem Biophys Res Commun, vol. 158, no. 3, Feb. 1989, pp. 943–50. Pubmed, doi:10.1016/0006-291x(89)92813-1.
Montefiori DC, Pellegrino MG, Robinson WE, Engle K, Field M, Mitchell WM, Gillespie DH. Inhibition of HIV-1 proviral DNA synthesis and RNA accumulation by mismatched dsRNA. Biochem Biophys Res Commun. 1989 Feb 15;158(3):943–950.
Journal cover image

Published In

Biochem Biophys Res Commun

DOI

ISSN

0006-291X

Publication Date

February 15, 1989

Volume

158

Issue

3

Start / End Page

943 / 950

Location

United States

Related Subject Headings

  • Zidovudine
  • Transcription, Genetic
  • T-Lymphocytes
  • Recombinant Proteins
  • RNA, Viral
  • RNA, Double-Stranded
  • Polyribonucleotides
  • Poly U
  • Poly I-C
  • Nucleic Acid Hybridization