Skip to main content
Journal cover image

In vitro evaluation of mismatched double-stranded RNA (ampligen) for combination therapy in the treatment of acquired immunodeficiency syndrome.

Publication ,  Journal Article
Montefiori, DC; Robinson, WE; Mitchell, WM
Published in: AIDS Res Hum Retroviruses
April 1989

Multiple drug effect analyses with mismatched double-stranded RNA (mismatched dsRNA or Ampligen) as a core drug were performed to identify other agents and mechanisms through which mismatched dsRNA may potentiate effective therapeutic intervention in human immunodeficiency virus (HIV) infection. Antiviral activities were defined by a microtiter infection assay utilizing MT-2 cells as targets and HTLV-III-B produced in H9 cells as a virus source. The scope of agents tested included rIFN-alpha A, rIFN-beta Ser 17, and rIFN-gamma as cytokines; azidothymidine and phosphonoformate (Foscarnet) as inhibitors of reverse transcription; ribavirin as a putative inhibitor of proper HIV mRNA capping; amphotericin B as a lipophile; and castanospermine as a glycoprotein processing (glucosidase I) inhibitor. Separately, each drug demonstrated dose-dependent anti-HIV activity and, when used in combination with mismatched dsRNA, demonstrated synergism. Although mismatched dsRNA was synergistic with all three IFNs for anti-HIV activity in microtiter infection assays, it did not potentiate the transient inhibition of virus production observed for IFN in cultures of H9/HTLV-III-B cells. The results of these studies suggest that the pleiotropic activities of dsRNAs differ from those of IFN and may provide synergism in combination therapy with a wide range of antiviral drugs for the treatment of the acquired immunodeficiency syndrome (AIDS).

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

AIDS Res Hum Retroviruses

DOI

ISSN

0889-2229

Publication Date

April 1989

Volume

5

Issue

2

Start / End Page

193 / 203

Location

United States

Related Subject Headings

  • Zidovudine
  • Virology
  • Ribavirin
  • Recombinant Proteins
  • RNA, Double-Stranded
  • Phosphonoacetic Acid
  • Microbial Sensitivity Tests
  • Interferons
  • Interferon-gamma
  • Interferon-beta
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Montefiori, D. C., Robinson, W. E., & Mitchell, W. M. (1989). In vitro evaluation of mismatched double-stranded RNA (ampligen) for combination therapy in the treatment of acquired immunodeficiency syndrome. AIDS Res Hum Retroviruses, 5(2), 193–203. https://doi.org/10.1089/aid.1989.5.193
Montefiori, D. C., W. E. Robinson, and W. M. Mitchell. “In vitro evaluation of mismatched double-stranded RNA (ampligen) for combination therapy in the treatment of acquired immunodeficiency syndrome.AIDS Res Hum Retroviruses 5, no. 2 (April 1989): 193–203. https://doi.org/10.1089/aid.1989.5.193.
Montefiori, D. C., et al. “In vitro evaluation of mismatched double-stranded RNA (ampligen) for combination therapy in the treatment of acquired immunodeficiency syndrome.AIDS Res Hum Retroviruses, vol. 5, no. 2, Apr. 1989, pp. 193–203. Pubmed, doi:10.1089/aid.1989.5.193.
Journal cover image

Published In

AIDS Res Hum Retroviruses

DOI

ISSN

0889-2229

Publication Date

April 1989

Volume

5

Issue

2

Start / End Page

193 / 203

Location

United States

Related Subject Headings

  • Zidovudine
  • Virology
  • Ribavirin
  • Recombinant Proteins
  • RNA, Double-Stranded
  • Phosphonoacetic Acid
  • Microbial Sensitivity Tests
  • Interferons
  • Interferon-gamma
  • Interferon-beta