Natural killer cell infection and inactivation in vitro by the human immunodeficiency virus.

Published

Journal Article

Cytolytic activity of human mononuclear peripheral blood leukocytes from healthy donors, cultured in interleukin-2 conditioned medium, was abrogated by in vitro infection with the lymphadenopathy associated virus (LAV) isolate of the human immunodeficiency virus (HIV). Although viral antigens are not expressed in cultured cells until 14 days postinfection, cytolytic activity was lost as early as 3 days after infection. Loss of cytolytic function was not a result of the release of suppressive factors from either infected cells or uninfected CEM cells since supernatants from neither infected cultures nor CEM cell cultures had any inhibitory effects on the function of uninfected cells. Cultured lymphocytes expressing Leu 11b were also shown to express HIV antigens via immunofluorescence after 14 days in culture. These results suggest that natural killer (NK) cells, as defined by expression of Leu 11b, were infected by HIV in vitro and the loss of lytic function was likely a direct consequence of that infection.

Full Text

Duke Authors

Cited Authors

  • Robinson, WE; Mitchell, WM; Chambers, WH; Schuffman, SS; Montefiori, DC; Oeltmann, TN

Published Date

  • May 1988

Published In

Volume / Issue

  • 19 / 5

Start / End Page

  • 535 - 540

PubMed ID

  • 3286479

Pubmed Central ID

  • 3286479

International Standard Serial Number (ISSN)

  • 0046-8177

Digital Object Identifier (DOI)

  • 10.1016/s0046-8177(88)80200-4

Language

  • eng

Conference Location

  • United States