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Macrophage colony-stimulating factor can modulate immune responses and attract dendritic cells in vivo.

Publication ,  Journal Article
Kim, JJ; Yang, JS; Lee, DJ; Wilson, DM; Nottingham, LK; Morrison, L; Tsai, A; Oh, J; Dang, K; Dentchev, T; Agadjanyan, MG; Sin, JI ...
Published in: Hum Gene Ther
January 20, 2000

Studies have indicated that professional APCs in the periphery, such as dendritic cells and macrophages, play an important role in initiating DNA vaccine-specific immune responses. To engineer the immune response induced by DNA vaccines in vivo we investigated the modulatory effects of codelivering growth factor genes for the hematopoietic APCs along with DNA vaccines. Specifically, we examined the effects on the antigen-specific immune responses following the codelivery of the gene expression cassettes for M-CSF, G-CSF, and GM-CSF along with HIV-1 DNA immunogen constructs. We observed that coimmunization with GM-CSF increased the antibody response and resulted in a significant enhancement of lymphoproliferative response. Furthermore, among all coinjection combinations, we found that M-CSF coinjections resulted in a high level of CTL enhancement. This enhancement of CTL responses observed from the coinjection with M-CSF was CD8+ T cell dependent and was associated with the presence of CD11c+ cells at the site of injection and with the antigen-specific induction of the beta-chemokine MIP-1beta, suggesting a role for this chemokine in CTL induction. These results suggest that hematopoietic growth factors should be further studied as potential adjuvants for in vivo modulators of immune responses.

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Published In

Hum Gene Ther

DOI

ISSN

1043-0342

Publication Date

January 20, 2000

Volume

11

Issue

2

Start / End Page

305 / 321

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • Promoter Regions, Genetic
  • Plasmids
  • Muscles
  • Mice, Inbred BALB C
  • Mice
  • Macrophage Colony-Stimulating Factor
  • Interleukin-4
  • Interleukin-12
 

Citation

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Kim, J. J., Yang, J. S., Lee, D. J., Wilson, D. M., Nottingham, L. K., Morrison, L., … Weiner, D. B. (2000). Macrophage colony-stimulating factor can modulate immune responses and attract dendritic cells in vivo. Hum Gene Ther, 11(2), 305–321. https://doi.org/10.1089/10430340050016049
Kim, J. J., J. S. Yang, D. J. Lee, D. M. Wilson, L. K. Nottingham, L. Morrison, A. Tsai, et al. “Macrophage colony-stimulating factor can modulate immune responses and attract dendritic cells in vivo.Hum Gene Ther 11, no. 2 (January 20, 2000): 305–21. https://doi.org/10.1089/10430340050016049.
Kim JJ, Yang JS, Lee DJ, Wilson DM, Nottingham LK, Morrison L, et al. Macrophage colony-stimulating factor can modulate immune responses and attract dendritic cells in vivo. Hum Gene Ther. 2000 Jan 20;11(2):305–21.
Kim, J. J., et al. “Macrophage colony-stimulating factor can modulate immune responses and attract dendritic cells in vivo.Hum Gene Ther, vol. 11, no. 2, Jan. 2000, pp. 305–21. Pubmed, doi:10.1089/10430340050016049.
Kim JJ, Yang JS, Lee DJ, Wilson DM, Nottingham LK, Morrison L, Tsai A, Oh J, Dang K, Dentchev T, Agadjanyan MG, Sin JI, Chalian AA, Weiner DB. Macrophage colony-stimulating factor can modulate immune responses and attract dendritic cells in vivo. Hum Gene Ther. 2000 Jan 20;11(2):305–321.
Journal cover image

Published In

Hum Gene Ther

DOI

ISSN

1043-0342

Publication Date

January 20, 2000

Volume

11

Issue

2

Start / End Page

305 / 321

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • T-Lymphocytes, Cytotoxic
  • Promoter Regions, Genetic
  • Plasmids
  • Muscles
  • Mice, Inbred BALB C
  • Mice
  • Macrophage Colony-Stimulating Factor
  • Interleukin-4
  • Interleukin-12