Engineering DNA vaccines via co-delivery of co-stimulatory molecule genes.
DNA immunization has been investigated as a potential immunization strategy against infectious diseases and cancer. To enhance a DNA vaccine's ability to induce CTL response in vivo, we co-administered CD80 and CD86 expression cassettes along with HIV-1 immunogens. This manipulation resulted in a dramatic increase in MHC class I-restricted and CD8+ T-cell-dependent CTL responses in both mice and chimpanzees. This strategy of engineering vaccine producing cells to be more efficient T-cell activators could be an important tool for optimizing antigen-specific T-cell-mediated immune responses in the pursuit of more rationally designed vaccines and immune therapies.
Kim, JJ; Nottingham, LK; Wilson, DM; Bagarazzi, ML; Tsai, A; Morrison, LD; Javadian, A; Chalian, AA; Agadjanyan, MG; Weiner, DB
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