Engineering DNA vaccines via co-delivery of co-stimulatory molecule genes.
Journal Article (Journal Article)
DNA immunization has been investigated as a potential immunization strategy against infectious diseases and cancer. To enhance a DNA vaccine's ability to induce CTL response in vivo, we co-administered CD80 and CD86 expression cassettes along with HIV-1 immunogens. This manipulation resulted in a dramatic increase in MHC class I-restricted and CD8+ T-cell-dependent CTL responses in both mice and chimpanzees. This strategy of engineering vaccine producing cells to be more efficient T-cell activators could be an important tool for optimizing antigen-specific T-cell-mediated immune responses in the pursuit of more rationally designed vaccines and immune therapies.
Full Text
Duke Authors
Cited Authors
- Kim, JJ; Nottingham, LK; Wilson, DM; Bagarazzi, ML; Tsai, A; Morrison, LD; Javadian, A; Chalian, AA; Agadjanyan, MG; Weiner, DB
Published Date
- November 1998
Published In
Volume / Issue
- 16 / 19
Start / End Page
- 1828 - 1835
PubMed ID
- 9795388
International Standard Serial Number (ISSN)
- 0264-410X
Digital Object Identifier (DOI)
- 10.1016/s0264-410x(98)00177-7
Language
- eng
Conference Location
- Netherlands