CD86 confers and directs MHC class I restricted CTL induction
The complex process of CTL induction is only partially understood. Activation of T cells requires both TCR-specific ligation by direct contact with peptide antigen-MHC complexes and co-ligation of the B7 family of ligands with CD28/CTLA-4 on the T cell surface. We investigated the role of the CD80 and CD86 molecules in the amplification of in vivo immune responses. We developed expression cassettes for CD80 and CD86 and analyzed their ability to function as in vivo modulators of the immune response induced by DNA immunization cassettes against HFV-1 antigens by co-immunizing them into mice. We observed that co-immunization with CD86, not CD80 expression cassettes, resulted in a dramatic increase in cytotoxic T lymphocyte response which was both MHC class I-restricted and CD8+ T cell-dependent. Using bone marrow chimeric animals, we found that the MHC class I-restriction of CTL was determined to be under the providence of the CD86, even when this molecule and antigen were displayed on a non-professional antigen presenting cell background. This study defines a role of CD86 in CTL immune induction and raises, new questions about the presumed function of CD80 in the generation of antigen specific immunity.
Kim, JJ; Agadjanyan, MG; Trivedi, N; Wilson, DM; Morrison, LD; Maldonado, MA; Williams, WV; Weiner, DB
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