A new face on apoptosis: death-associated protein 3 and PDCD9 are mitochondrial ribosomal proteins.

Journal Article (Journal Article)

Two proteins known to be involved in promoting apoptosis in mammalian cells have been identified as components of the mammalian mitochondrial ribosome. Proteolytic digestion of whole mitochondrial ribosomal subunits followed by analysis of the peptides present using liquid chromatography-tandem mass spectrometry revealed that the proapoptotic proteins, death-associated protein 3 (DAP3) and the programmed cell death protein 9, are both components of the mitochondrial ribosome. DAP3 has motifs characteristic of guanine nucleotide binding proteins and is probably the protein that accounts for the nucleotide binding activity of mammalian mitochondrial ribosomes. The observations reported here implicate mitochondrial protein synthesis as a major component in cellular apoptotic signaling pathways.

Full Text

Duke Authors

Cited Authors

  • Cavdar Koc, E; Ranasinghe, A; Burkhart, W; Blackburn, K; Koc, H; Moseley, A; Spremulli, LL

Published Date

  • March 9, 2001

Published In

Volume / Issue

  • 492 / 1-2

Start / End Page

  • 166 - 170

PubMed ID

  • 11248257

International Standard Serial Number (ISSN)

  • 0014-5793

Digital Object Identifier (DOI)

  • 10.1016/s0014-5793(01)02250-5


  • eng

Conference Location

  • England