Sensitive analysis of [D-Pen2,5]enkephalin in rat serum by capillary electrophoresis and laser-induced fluorescence detection.

Journal Article (Journal Article)

A highly sensitive analytical method based on capillary zone electrophoresis (CZE) coupled with a laser-induced fluorescence (LIF) detector was explored for the analysis of [D-Pen2,5]enkephalin (DPDPE) in rat serum. DPDPE and the internal standard Phe-Leu-Glu-Glu-Ile (P9396) were extracted from serum samples with C18 solid-phase extraction disk cartridges, followed by derivatization with tetramethylrhodamine-5-isothiocyanate (TRITC) isomer G before introduction onto the capillary column. Complete resolution of DPDPE and the internal standard from other serum components was achieved within 20 min on a 140 cm x 50 microns I.D. capillary column with borate buffer (25 mM. pH 8.3). With the current method, it is possible to detect 1.3E-18 mol of DPDPE on column. The results suggest that CZE-LIF is a promising method for the sensitive and specific quantitation of therapeutic peptides in biological matrices.

Full Text

Duke Authors

Cited Authors

  • Chen, C; Jeffery, D; Jorgenson, JW; Moseley, MA; Pollack, GM

Published Date

  • September 12, 1997

Published In

Volume / Issue

  • 697 / 1-2

Start / End Page

  • 149 - 162

PubMed ID

  • 9342665

International Standard Serial Number (ISSN)

  • 1387-2273

Digital Object Identifier (DOI)

  • 10.1016/s0378-4347(97)00062-5


  • eng

Conference Location

  • Netherlands