Determination of bioactive peptides using capillary zone electrophoresis/mass spectrometry.


Journal Article

Mixtures of bioactive peptides have been analyzed by capillary zone electrophoresis/mass spectrometry (CZE/MS) using an on-line coaxial continuous-flow fast atom bombardment interface. High separation efficiencies (up to 410,000 theoretical plates) were obtained from low femtomole levels of peptides. The analysis of basic peptides was accomplished by using aminopropyl-silylated CZE columns to minimize zone broadening due to adsorption effects. CZE/MS/MS data were acquired from femtomole levels of peptides in electrophoretic real time.

Full Text

Duke Authors

Cited Authors

  • Moseley, MA; Deterding, LJ; Tomer, KB; Jorgenson, JW

Published Date

  • January 15, 1991

Published In

Volume / Issue

  • 63 / 2

Start / End Page

  • 109 - 114

PubMed ID

  • 1812793

Pubmed Central ID

  • 1812793

International Standard Serial Number (ISSN)

  • 0003-2700

Digital Object Identifier (DOI)

  • 10.1021/ac00002a005


  • eng

Conference Location

  • United States