Continuous flow fast atom bombardment with packed microcolumns: A comparison of precolumn versus coaxial matrix delivery
Published
Journal Article
The effect of adding glycerol to the mobile phase on the chromatographic separation of peptides has been investigated using a continuous flow fast atom bombardment (CFFAB) interface coupled with commercial packed microcolumns (25 cm × 320 μm.i.d.). In a comparative study using a UV detector, it was found that chromatographic peak broadening progressively increased with increasing percentage of glycerol in the mobile phase. In the liquid chromatographic FAB mass spectrometric analysis, this effect is compounded by the dynamic mixing of the column effluent on the probe. Improvements of 25-155% in the overall separation efficiencies were obtained by introducing the matrix independently to the probe tip via a coaxial arrangement. Application of this coaxial CFFAB is demonstrated by the analysis of peptide mixtures and tryptic digests. © 1990 American Society for Mass Spectrometry.
Full Text
Duke Authors
Cited Authors
- Pleasance, S; Thibault, P; Moseley, MA; Deterding, LJ; Tomer, KB; Jorgenson, JW
Published Date
- January 1, 1990
Published In
Volume / Issue
- 1 / 4
Start / End Page
- 312 - 319
Electronic International Standard Serial Number (EISSN)
- 1879-1123
International Standard Serial Number (ISSN)
- 1044-0305
Digital Object Identifier (DOI)
- 10.1016/1044-0305(90)85007-9
Citation Source
- Scopus