Capillary-zone electrophoresis/fast-atom bombardment mass spectrometry: design of an on-line coaxial continuous-flow interface.

Published

Journal Article

An on-line coaxial continuous-flow capillary-zone electrophoresis/fast-atom bombardment mass spectrometry (CZE/FAB-MS) interface is described. This interface is shown to be capable of acquiring mass spectra in an on-line fashion from low femtomole amounts of peptides while maintaining high (hundreds of thousands of plates) electrophoretic separation efficiencies. Active electrophoretic transport of the analytes directly to the FAB probe tip obviates the need for a transfer line from the end of the CZE capillary to this point, and thereby precludes the zone broadening that would otherwise occur both within such a transfer line and in the connections between the CZE column and the transfer line. The capability of acquiring an on-line tandem mass spectrometry (MS/MS) spectrum of an electrophoretically separated analyte using this interface is also demonstrated.

Full Text

Duke Authors

Cited Authors

  • Moseley, MA; Deterding, LJ; Tomer, KB; Jorgenson, JW

Published Date

  • March 1989

Published In

Volume / Issue

  • 3 / 3

Start / End Page

  • 87 - 93

PubMed ID

  • 2520229

Pubmed Central ID

  • 2520229

International Standard Serial Number (ISSN)

  • 0951-4198

Digital Object Identifier (DOI)

  • 10.1002/rcm.1290030310

Language

  • eng

Conference Location

  • England