Convenient vectors for cloning and sequencing EcoRI and HindIII fragments.

Published

Journal Article

The polylinker regions of plasmid pUC and bacteriophage M13mp vectors have been specifically modified to provide alternative positions for cloning and reexcising EcoRI and HindIII fragments; the EcoRI and HindIII sites have been moved internal to BamHI and Bg/II sites. The location of EcoRI and HindIII sites in these HinEco vectors allows either selective linearization or excision of the cloned fragments at unique flanking sites.

Full Text

Cited Authors

  • Edenberg, HJ; Moss, LG; Rutter, WJ

Published Date

  • January 1987

Published In

Volume / Issue

  • 58 / 2-3

Start / End Page

  • 297 - 298

PubMed ID

  • 2828191

Pubmed Central ID

  • 2828191

Electronic International Standard Serial Number (EISSN)

  • 1879-0038

International Standard Serial Number (ISSN)

  • 0378-1119

Digital Object Identifier (DOI)

  • 10.1016/0378-1119(87)90384-2

Language

  • eng