Regionally distinct stoichiometry for N-methyl-D-aspartate receptor domains in brain.

Journal Article

A stoichiometric analysis of N-methyl-D-aspartate (NMDA) receptor binding was conducted using [3H]dizocilpine, [3H]dichlorokynurenic acid and [3H]CGP39653, and membranes from various brain regions of rats. The ratio of the density of [3H]CGP39653 binding to [3H]dizocilpine binding was > 1 in frontal cortex and hippocampus, approximately 1 in striatum and spinal cord and < 1 in cerebellum. When [3H]dichlorokynurenic acid binding was compared with [3H]dizocilpine binding, the ratios were > 1 in frontal cortex, hippocampus and striatum, 3-4 in cerebellum, and approximately 2 in spinal cord. These observations suggest that a single channel complex may have more than one binding site for NMDA and/or glycine and that the stoichiometry between the binding domains of the NMDA receptor varies regionally.

Full Text

Duke Authors

Cited Authors

  • Matsunaga, T; Mukhin, AG; London, ED

Published Date

  • February 29, 1996

Published In

Volume / Issue

  • 7 / 3

Start / End Page

  • 833 - 837

PubMed ID

  • 8733756

International Standard Serial Number (ISSN)

  • 0959-4965

Language

  • eng

Conference Location

  • England