Regionally distinct stoichiometry for N-methyl-D-aspartate receptor domains in brain.
Journal Article (Journal Article)
A stoichiometric analysis of N-methyl-D-aspartate (NMDA) receptor binding was conducted using [3H]dizocilpine, [3H]dichlorokynurenic acid and [3H]CGP39653, and membranes from various brain regions of rats. The ratio of the density of [3H]CGP39653 binding to [3H]dizocilpine binding was > 1 in frontal cortex and hippocampus, approximately 1 in striatum and spinal cord and < 1 in cerebellum. When [3H]dichlorokynurenic acid binding was compared with [3H]dizocilpine binding, the ratios were > 1 in frontal cortex, hippocampus and striatum, 3-4 in cerebellum, and approximately 2 in spinal cord. These observations suggest that a single channel complex may have more than one binding site for NMDA and/or glycine and that the stoichiometry between the binding domains of the NMDA receptor varies regionally.
Full Text
Duke Authors
Cited Authors
- Matsunaga, T; Mukhin, AG; London, ED
Published Date
- February 29, 1996
Published In
Volume / Issue
- 7 / 3
Start / End Page
- 833 - 837
PubMed ID
- 8733756
International Standard Serial Number (ISSN)
- 0959-4965
Digital Object Identifier (DOI)
- 10.1097/00001756-199602290-00036
Language
- eng
Conference Location
- England