Muscimol-induced stimulation of diazepam binding in intensely washed membranes of the rat brain
Using intensely washed 1% Triton X-100 treated rat brain membranes the EC50 of muscimol for 3H-diazepam (0.5 nM) specific binding was found to be equal to 12.2 nM. The IC50 of muscimol for 3H-muscimol (6 nM) specific binding was equal to 8.8 nM. Two types of binding sites for 3H-muscimol were detected in 1% Triton X-100 treated membranes with the K(d) values of 1.8 nM (Type 1) and 13 nM (Type 2). Similarly of the EC50 of muscimol for diazepam binding and of the IC50 of muscimol for muscimol binding site indicates that the stimulation of benzodiazepine receptor binding is mediated through type 2 of the postsynaptic muscimol binding sites.