OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.
Publication
, Journal Article
Murdoch, DM; Staats, JS; Weinhold, KJ
Published in: Cytometry A
April 2012
This panel was optimized for the enumeration and phenotypic characterization of T regulatory cells (Tregs) within the CD4⁺ T-cell pool using human peripheral blood mononuclear cells (PBMC) using intranuclear and intracellular staining methods. The panel was optimized for HIV⁺ clinical trial specimens through the use of HIV-infected and normal donor PBMC. Because the panel is to be used in the context of testing cryopreserved PBMC obtained from multiple sites participating in clinical trials, it was essential to develop an assay that performed well using cryopreserved PBMC. Other tissue types have not been tested.
Duke Scholars
Published In
Cytometry A
DOI
EISSN
1552-4930
Publication Date
April 2012
Volume
81
Issue
4
Start / End Page
281 / 283
Location
United States
Related Subject Headings
- T-Lymphocytes, Regulatory
- Phenotype
- Leukocytes, Mononuclear
- Immunology
- Humans
- HIV Infections
- Flow Cytometry
- 3201 Cardiovascular medicine and haematology
- 0601 Biochemistry and Cell Biology
Citation
APA
Chicago
ICMJE
MLA
NLM
Murdoch, D. M., Staats, J. S., & Weinhold, K. J. (2012). OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry. Cytometry A, 81(4), 281–283. https://doi.org/10.1002/cyto.a.22024
Murdoch, David M., Janet S. Staats, and Kent J. Weinhold. “OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.” Cytometry A 81, no. 4 (April 2012): 281–83. https://doi.org/10.1002/cyto.a.22024.
Murdoch DM, Staats JS, Weinhold KJ. OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry. Cytometry A. 2012 Apr;81(4):281–3.
Murdoch, David M., et al. “OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.” Cytometry A, vol. 81, no. 4, Apr. 2012, pp. 281–83. Pubmed, doi:10.1002/cyto.a.22024.
Murdoch DM, Staats JS, Weinhold KJ. OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry. Cytometry A. 2012 Apr;81(4):281–283.
Published In
Cytometry A
DOI
EISSN
1552-4930
Publication Date
April 2012
Volume
81
Issue
4
Start / End Page
281 / 283
Location
United States
Related Subject Headings
- T-Lymphocytes, Regulatory
- Phenotype
- Leukocytes, Mononuclear
- Immunology
- Humans
- HIV Infections
- Flow Cytometry
- 3201 Cardiovascular medicine and haematology
- 0601 Biochemistry and Cell Biology