OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.

Journal Article (Journal Article)

This panel was optimized for the enumeration and phenotypic characterization of T regulatory cells (Tregs) within the CD4⁺ T-cell pool using human peripheral blood mononuclear cells (PBMC) using intranuclear and intracellular staining methods. The panel was optimized for HIV⁺ clinical trial specimens through the use of HIV-infected and normal donor PBMC. Because the panel is to be used in the context of testing cryopreserved PBMC obtained from multiple sites participating in clinical trials, it was essential to develop an assay that performed well using cryopreserved PBMC. Other tissue types have not been tested.

Full Text

Duke Authors

Cited Authors

  • Murdoch, DM; Staats, JS; Weinhold, KJ

Published Date

  • April 2012

Published In

Volume / Issue

  • 81 / 4

Start / End Page

  • 281 - 283

PubMed ID

  • 22319016

Pubmed Central ID

  • PMC3352600

Electronic International Standard Serial Number (EISSN)

  • 1552-4930

Digital Object Identifier (DOI)

  • 10.1002/cyto.a.22024


  • eng

Conference Location

  • United States