Skip to main content

Involvement of an ATP-dependent peptide chaperone in cross-presentation after DNA immunization.

Publication ,  Journal Article
Kumaraguru, U; Rouse, RJ; Nair, SK; Bruce, BD; Rouse, BT
Published in: J Immunol
July 15, 2000

Immunization with plasmid DNA holds promise as a vaccination strategy perhaps useful in situations that currently lack vaccines, since the major means of immune induction may differ from more conventional approach. In the present study, we demonstrate that exposure of macrophages to plasmid DNA encoding viral proteins or OVA generates Ag-specific material that, when presented in vitro by dendritic cells to naive T cells, induces primary CTL response or elicits IL-2 production from an OVA peptide-specific T-T hybridoma. The immunogenic material released was proteinaceous in nature, free of apoptotic bodies, and had an apparent m.w. much larger than a 9-11-aa CTL-recognizable peptide. The macrophage-released factor(s) specifically required a hydrolyzable ATP substrate and was inhibited by procedures that removed or hydrolyzed ATP; in addition, anti-heat-shock protein 70 antiserum abrogated the activity to a large extent. These results indicate the possible involvement of a heat-shock protein 70-linked peptide chaperone in a cross-priming method of immune induction by DNA vaccination. Such a cross-priming process may represent a principal mechanism by which plasmid DNA delivered to cells such as myocytes effectively shuttle Ag to DC or other APC to achieve CTL induction in vivo.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

July 15, 2000

Volume

165

Issue

2

Start / End Page

750 / 759

Location

United States

Related Subject Headings

  • Viral Envelope Proteins
  • Vaccines, DNA
  • Tumor Cells, Cultured
  • Transfection
  • Plasmids
  • Peptide Fragments
  • Ovalbumin
  • Oligopeptides
  • Molecular Chaperones
  • Mice, Inbred C57BL
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Kumaraguru, U., Rouse, R. J., Nair, S. K., Bruce, B. D., & Rouse, B. T. (2000). Involvement of an ATP-dependent peptide chaperone in cross-presentation after DNA immunization. J Immunol, 165(2), 750–759. https://doi.org/10.4049/jimmunol.165.2.750
Kumaraguru, U., R. J. Rouse, S. K. Nair, B. D. Bruce, and B. T. Rouse. “Involvement of an ATP-dependent peptide chaperone in cross-presentation after DNA immunization.J Immunol 165, no. 2 (July 15, 2000): 750–59. https://doi.org/10.4049/jimmunol.165.2.750.
Kumaraguru U, Rouse RJ, Nair SK, Bruce BD, Rouse BT. Involvement of an ATP-dependent peptide chaperone in cross-presentation after DNA immunization. J Immunol. 2000 Jul 15;165(2):750–9.
Kumaraguru, U., et al. “Involvement of an ATP-dependent peptide chaperone in cross-presentation after DNA immunization.J Immunol, vol. 165, no. 2, July 2000, pp. 750–59. Pubmed, doi:10.4049/jimmunol.165.2.750.
Kumaraguru U, Rouse RJ, Nair SK, Bruce BD, Rouse BT. Involvement of an ATP-dependent peptide chaperone in cross-presentation after DNA immunization. J Immunol. 2000 Jul 15;165(2):750–759.

Published In

J Immunol

DOI

ISSN

0022-1767

Publication Date

July 15, 2000

Volume

165

Issue

2

Start / End Page

750 / 759

Location

United States

Related Subject Headings

  • Viral Envelope Proteins
  • Vaccines, DNA
  • Tumor Cells, Cultured
  • Transfection
  • Plasmids
  • Peptide Fragments
  • Ovalbumin
  • Oligopeptides
  • Molecular Chaperones
  • Mice, Inbred C57BL