Heparan sulfate modulate allogeneic T cell responses stimulated by dendritic cells

Conference Paper

Heparan sulfate (HS) is a biologically active glycosaminoglycan which is released from cell surfaces or extracellular matrices under the circumstances of tissue injury or inflammation. We have reported previously that HS modulates T cell immune responses by direct activation of macrophages. Here, we examined the effect of free HS on dendritic cells (DC) as accessory cells in allogeneic mixed lymphocyte reactions. A T cell-enriched fraction of splenocytes from BALB/c mice was co-cultured with DC from C57BL/6 mice. DC were isolated from either spleen cells and cultured bone marrow cells with GM-CSF. Under suboptimal conditions (supplemented with 1% mouse serum), 103-104 DC treated with HS induced a greater allogeneic response than untreated DC. Counts per million at 4 days culture were as follows: splenic DC (103), 7751.3 +/- 1635.4 with HS vs. 1390.3 +/- 264.2 without, bone marrow DC (5×103), 6285.8 +/- 860.7 with HS vs. 1959.3 +/- 204.5 without. These results suggest that free HS may modulate cellular immune responses in the microenvironment of local inflammatory sites by providing inflammatory signals that modify the function of DC.

Duke Authors

Cited Authors

  • Kodaira, Y; Wrenshall, LE; Nair, S; Gilboa, E; Platt, JL

Published Date

  • January 1, 1998

Published In

Volume / Issue

  • 12 / 4

Start / End Page

  • A582 -

International Standard Serial Number (ISSN)

  • 0892-6638