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Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells

Publication ,  Journal Article
Wong, JV; Yao, G; Nevins, JR; You, L
Published in: Methods in Enzymology
May 30, 2011

Perturbations from environmental, genetic, and pharmacological sources can generate heterogeneous biological responses, even in genetically identical cells. Although these differences have important consequences on cell physiology and survival, they are often subsumed in measurements that average over the population. Here, we describe in detail how variability in adenoviral-mediated gene expression provides an effective means to map dose responses of signaling pathways. Cellcell variability is inherent in gene delivery methods used in cell biology, which makes this approach adaptable to many existing experimental systems. We also discuss strategies to quantify biologically relevant inputs and outputs. © 2011 Elsevier Inc. All rights reserved.

Duke Scholars

Published In

Methods in Enzymology

DOI

EISSN

1557-7988

ISSN

0076-6879

Publication Date

May 30, 2011

Volume

497

Start / End Page

221 / 237

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology
 

Citation

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ICMJE
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Wong, J. V., Yao, G., Nevins, J. R., & You, L. (2011). Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells. Methods in Enzymology, 497, 221–237. https://doi.org/10.1016/B978-0-12-385075-100010-X
Wong, J. V., G. Yao, J. R. Nevins, and L. You. “Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells.” Methods in Enzymology 497 (May 30, 2011): 221–37. https://doi.org/10.1016/B978-0-12-385075-100010-X.
Wong JV, Yao G, Nevins JR, You L. Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells. Methods in Enzymology. 2011 May 30;497:221–37.
Wong, J. V., et al. “Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells.” Methods in Enzymology, vol. 497, May 2011, pp. 221–37. Scopus, doi:10.1016/B978-0-12-385075-100010-X.
Wong JV, Yao G, Nevins JR, You L. Using noisy gene expression mediated by engineered adenovirus to probe signaling dynamics in mammalian cells. Methods in Enzymology. 2011 May 30;497:221–237.

Published In

Methods in Enzymology

DOI

EISSN

1557-7988

ISSN

0076-6879

Publication Date

May 30, 2011

Volume

497

Start / End Page

221 / 237

Related Subject Headings

  • Biochemistry & Molecular Biology
  • 3101 Biochemistry and cell biology
  • 0601 Biochemistry and Cell Biology