The interaction of RB with E2F coincides with an inhibition of the transcriptional activity of E2F.


Journal Article

Recent experiments have shown that the E2F transcription factor is in a complex with the RB1 gene product. The E2F-pRB complex can be reconstituted in an in vitro assay using a GST-RB fusion protein isolated from Escherichia coli. This interaction is dependent on pRB sequences involved in E1A/T-antigen binding as well as carboxy-terminal pRB sequences that are not necessary for E1A/T binding. Moreover, reconstitution assays reveal a requirement for an accessory factor, in addition to E2F and pRB, for formation of the E2F-pRB complex. Assays of transcription from the adenovirus E2 promoter in transfection experiments demonstrate that formation of the complex containing pRB and E2F coincides with an inhibition of E2F-dependent transcriptional activity. A mutant pRB protein that does not associate with E2F does not inhibit transcription. We conclude that as a consequence of its interaction with E2F, pRB may regulate the transcriptional function of the E2F factor.

Full Text

Cited Authors

  • Hiebert, SW; Chellappan, SP; Horowitz, JM; Nevins, JR

Published Date

  • February 1, 1992

Published In

Volume / Issue

  • 6 / 2

Start / End Page

  • 177 - 185

PubMed ID

  • 1531329

Pubmed Central ID

  • 1531329

Electronic International Standard Serial Number (EISSN)

  • 1549-5477

International Standard Serial Number (ISSN)

  • 0890-9369

Digital Object Identifier (DOI)

  • 10.1101/gad.6.2.177


  • eng