Contamination with E1A-positive wild-type adenovirus accounts for species-specific stimulation of islet cell proliferation by CCK: a cautionary note.
Published
Journal Article
We have previously reported that adenovirus-mediated expression of preprocholecystokin (CCK) stimulates human and mouse islet cell proliferation. In follow-up studies, we became concerned that the CCK adenovirus might have been contaminated with a wild-type E1A-containing adenovirus. Here we show conclusively that the proliferative effects reported in the original paper in mouse and human islets were not due to CCK expression but rather to a contaminating E1A-expressing wild-type adenovirus. We also show, however, that CCK expression does have a proliferative effect in rat islets. We hope that our report of the steps taken to detect the wild-type virus contamination, and purification of the contributing viral stocks, will be helpful to other investigators, and that our experience will serve as a cautionary tale for use of adenovirus vectors, especially for studies on cellular replication.
Full Text
Duke Authors
Cited Authors
- Lavine, JA; Raess, PW; Davis, DB; Rabaglia, ME; Presley, BK; Keller, MP; Beinfeld, MC; Kopin, AS; Newgard, CB; Attie, AD
Published Date
- February 2010
Published In
Volume / Issue
- 24 / 2
Start / End Page
- 464 - 467
PubMed ID
- 20081104
Pubmed Central ID
- 20081104
Electronic International Standard Serial Number (EISSN)
- 1944-9917
Digital Object Identifier (DOI)
- 10.1210/me.2009-0384
Language
- eng
Conference Location
- United States