Contamination with E1A-positive wild-type adenovirus accounts for species-specific stimulation of islet cell proliferation by CCK: a cautionary note.

Published

Journal Article

We have previously reported that adenovirus-mediated expression of preprocholecystokin (CCK) stimulates human and mouse islet cell proliferation. In follow-up studies, we became concerned that the CCK adenovirus might have been contaminated with a wild-type E1A-containing adenovirus. Here we show conclusively that the proliferative effects reported in the original paper in mouse and human islets were not due to CCK expression but rather to a contaminating E1A-expressing wild-type adenovirus. We also show, however, that CCK expression does have a proliferative effect in rat islets. We hope that our report of the steps taken to detect the wild-type virus contamination, and purification of the contributing viral stocks, will be helpful to other investigators, and that our experience will serve as a cautionary tale for use of adenovirus vectors, especially for studies on cellular replication.

Full Text

Duke Authors

Cited Authors

  • Lavine, JA; Raess, PW; Davis, DB; Rabaglia, ME; Presley, BK; Keller, MP; Beinfeld, MC; Kopin, AS; Newgard, CB; Attie, AD

Published Date

  • February 2010

Published In

Volume / Issue

  • 24 / 2

Start / End Page

  • 464 - 467

PubMed ID

  • 20081104

Pubmed Central ID

  • 20081104

Electronic International Standard Serial Number (EISSN)

  • 1944-9917

Digital Object Identifier (DOI)

  • 10.1210/me.2009-0384

Language

  • eng

Conference Location

  • United States