Contamination with E1A-positive wild-type adenovirus accounts for species-specific stimulation of islet cell proliferation by CCK: a cautionary note.
We have previously reported that adenovirus-mediated expression of preprocholecystokin (CCK) stimulates human and mouse islet cell proliferation. In follow-up studies, we became concerned that the CCK adenovirus might have been contaminated with a wild-type E1A-containing adenovirus. Here we show conclusively that the proliferative effects reported in the original paper in mouse and human islets were not due to CCK expression but rather to a contaminating E1A-expressing wild-type adenovirus. We also show, however, that CCK expression does have a proliferative effect in rat islets. We hope that our report of the steps taken to detect the wild-type virus contamination, and purification of the contributing viral stocks, will be helpful to other investigators, and that our experience will serve as a cautionary tale for use of adenovirus vectors, especially for studies on cellular replication.
Duke Scholars
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Related Subject Headings
- Transfection
- Species Specificity
- Sequence Alignment
- Reverse Transcriptase Polymerase Chain Reaction
- Rats
- RNA, Messenger
- Protein Precursors
- Molecular Sequence Data
- Mice
- Islets of Langerhans
Citation
Published In
DOI
EISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Transfection
- Species Specificity
- Sequence Alignment
- Reverse Transcriptase Polymerase Chain Reaction
- Rats
- RNA, Messenger
- Protein Precursors
- Molecular Sequence Data
- Mice
- Islets of Langerhans