Peptide-mediated targeting of the islets of Langerhans.

Published

Journal Article

Strategies for restoring beta-cell function in diabetic patients would be greatly aided by the ability to target genes, proteins, or small molecules specifically to these cells. Furthermore, the ability to direct imaging agents specifically to beta-cells would facilitate diagnosis and monitoring of disease progression. To isolate ligands that can home to beta-cells in vivo, we have panned a random phage-displayed 20-mer peptide library on freshly isolated rat islets. We have isolated two 20-mer peptides that bind to islets ex vivo. One of these peptides preferentially homes to the islets of Langerhans in a normal rat with clear differentiation between the endocrine and exocrine cells of the pancreas. Furthermore, this peptide does not target beta-cells in a type 2 diabetes animal model, suggesting that the peptide can discriminate between glucose-stimulated insulin secretion-functional and -dysfunctional beta-cells.

Full Text

Duke Authors

Cited Authors

  • Samli, KN; McGuire, MJ; Newgard, CB; Johnston, SA; Brown, KC

Published Date

  • July 2005

Published In

Volume / Issue

  • 54 / 7

Start / End Page

  • 2103 - 2108

PubMed ID

  • 15983211

Pubmed Central ID

  • 15983211

International Standard Serial Number (ISSN)

  • 0012-1797

Digital Object Identifier (DOI)

  • 10.2337/diabetes.54.7.2103

Language

  • eng

Conference Location

  • United States