Out with the old, in with the new? Comparing methods for measuring protein degradation.

Published

Journal Article (Review)

Protein degradation is a critical factor in controlling cellular protein abundance. Here, we compare classical methods for determining protein degradation rates to a novel GFP (green fluorescent protein) fusion protein based method that assesses the intrinsic stability of cloned cDNA library products by flow cytometry [Yen et al. (2008) Science 322, 918]. While no method is perfect, we conclude that chimeric gene reporter approaches, though powerful, should be applied cautiously, due principally to GFP (or other reporter tag) interference with protein organelle targeting or incorporation into macromolecular assemblies, both of which cause spuriously high degradation rates.

Full Text

Duke Authors

Cited Authors

  • Yewdell, JW; Lacsina, JR; Rechsteiner, MC; Nicchitta, CV

Published Date

  • May 2011

Published In

Volume / Issue

  • 35 / 5

Start / End Page

  • 457 - 462

PubMed ID

  • 21476986

Pubmed Central ID

  • 21476986

Electronic International Standard Serial Number (EISSN)

  • 1095-8355

International Standard Serial Number (ISSN)

  • 1065-6995

Digital Object Identifier (DOI)

  • 10.1042/CBI20110055

Language

  • eng