Efficient cross-priming of antiviral CD8+ T cells by antigen donor cells is GRP94 independent.

Published

Journal Article

Cross-priming, the activation of naive CD8+ T cells by dendritic cells presenting Ags synthesized by other cells, is believed to play an important role in the generation of antiviral and antitumor responses. The molecular mechanism(s) underlying cross-priming remain poorly defined and highly controversial. GRP94 (gp96), an abundant endoplasmic reticulum chaperone with innate immune-activating capacity, has been widely reported to play a major role in cross-priming. In this study, we show that cells whose expression of GRP94 is silenced via transient or stable transfection with GRP94-directed small interfering RNAs demonstrate no reduction in their abilities to generate class I peptide complexes in cultured cells or to prime antiviral CD8+ T cell responses in vivo. In demonstrating the dispensability of GRP94, our finding points to the importance of alternative mechanisms for generation of class I peptide complexes from endogenous and exogenous Ags and immunogens.

Full Text

Duke Authors

Cited Authors

  • Lev, A; Dimberu, P; Das, SR; Maynard, JC; Nicchitta, CV; Bennink, JR; Yewdell, JW

Published Date

  • October 1, 2009

Published In

Volume / Issue

  • 183 / 7

Start / End Page

  • 4205 - 4210

PubMed ID

  • 19752220

Pubmed Central ID

  • 19752220

Electronic International Standard Serial Number (EISSN)

  • 1550-6606

Digital Object Identifier (DOI)

  • 10.4049/jimmunol.0901828

Language

  • eng

Conference Location

  • United States