Probing protein-protein interactions with chemical cross-linking and differential nano-LC-MS


Journal Article

Protein-protein interaction was investigated using chemical cross-linking and differential nano-LC-MS. The tryptic digestion mixtures were analyzed by nano-LC-MS. GGH-ecotin D137Y was used as a model protein and cross-linked by primary amine reactive cross-linker, disuccinimidyl suberate (DSS). A retrospective analysis of the wild-type ecotin X-ray crystal structure shows that the cross-linked residues are all on the dimeric interface.

Duke Authors

Cited Authors

  • Chu, F; Baker, P; Mahrus, S; Craik, CS; Rosser, M; Nicchitta, CV; Burlingame, AL

Published Date

  • December 1, 2002

Published In

  • Proceedings 50th Asms Conference on Mass Spectrometry and Allied Topics

Start / End Page

  • 289 - 290

Citation Source

  • Scopus