Spatiotemporal coupling between hippocampal acetylcholine release and theta oscillations in vivo.

Published

Journal Article

Both acetylcholine (ACh) and theta oscillations are important for learning and memory, but the dynamic interaction between these two processes remains unclear. Recent advances in amperometry techniques have revealed phasic ACh releases in vivo. However, it is unknown whether phasic ACh release co-occurs with theta oscillations. We investigated this issue in the CA1 region of urethane-anesthetized male rats using amperometric and electrophysiological recordings. We found that ACh release was highly correlated with the appearance of both spontaneous and induced theta oscillations. Moreover, the maximal ACh release was observed around or slightly above the pyramidal layer. Interestingly, such release lagged behind theta initiation by 25-60 s. The slow ACh release profile was matched by the slow firing rate increase of a subset of medial-septal low-firing-rate neurons. Together, these results establish, for the first time, the in vivo coupling between phasic ACh release and theta oscillations on spatiotemporal scales much finer than previously known. These findings also suggest that phasic ACh is not required for theta initiation and may instead operate synergistically with theta oscillations to promote neural plasticity in the service of learning and memory.

Full Text

Duke Authors

Cited Authors

  • Zhang, H; Lin, S-C; Nicolelis, MAL

Published Date

  • October 6, 2010

Published In

Volume / Issue

  • 30 / 40

Start / End Page

  • 13431 - 13440

PubMed ID

  • 20926669

Pubmed Central ID

  • 20926669

Electronic International Standard Serial Number (EISSN)

  • 1529-2401

Digital Object Identifier (DOI)

  • 10.1523/JNEUROSCI.1144-10.2010

Language

  • eng

Conference Location

  • United States