Relative increase in creatine kinase MB isoenzyme during reperfusion after myocardial infarction is method dependent

Published

Journal Article

We compared relative increases in creatine kinase (EC 2.7.3.2) MB isoenzyme (CK-MB) after reperfusion in myocardial infarction for four popular methods: electrophoresis, immunoinhibition, the 'Magic Lite' (Ciba-Corning) system, and the Stratus (Dade). In a method comparison study, we confirmed that all four methods correlated (r > 0.95). Electrophoresis demonstrated the greatest scatter about the regression line, immunoinhibition the least. For CK-MB quantities near each method's 'positive cutoff' indicating mycardial infarction, results by all methods agreed in 95% of samples. To characterize relative increases in CK-MB, we computer-fitted data obtained from each method for serial specimens collected from six acute myocardial infarction patients during myocardial reperfusion. Although for each individual patient the four methods appeared to exhibit parallelism, the methods differed significantly in terms describing their appearance rate, peak-time and falloff, and time-to-peak activity. Consistent with these data, we found that the relative CK-MB increases at various times after reperfusion, compared with baseline concentrations, are method-dependent. Therefore, when using CK-MB for indicating coronary patency, one must develop specific limits for each method utilized.

Duke Authors

Cited Authors

  • Christenson, RH; Clemmensen, P; Ohman, EM; Toffaletti, J; Silverman, LM; Grande, P; Vollmer, RT; Wagner, GS

Published Date

  • September 19, 1990

Published In

Volume / Issue

  • 36 / 8 I

Start / End Page

  • 1444 - 1449

International Standard Serial Number (ISSN)

  • 0009-9147

Citation Source

  • Scopus