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Aberrant FHIT mRNA transcripts are present in malignant and normal haematopoiesis, but absence of FHIT protein is restricted to leukaemia.

Publication ,  Journal Article
Peters, UR; Hasse, U; Oppliger, E; Tschan, M; Ong, ST; Rassool, FV; Borisch, B; Tobler, A; Fey, MF
Published in: Oncogene
January 7, 1999

Aberrant FHIT mRNA transcripts are present in malignant and normal haematopoiesis, but absence of FHIT protein is restricted to leukaemia Alterations of the recently cloned fragile histidine triad (FHIT) gene at chromosome 3p14.2 are frequent in a variety of solid tumours and cancer cell lines. Based on these findings, FHIT has been proposed as a putative tumour-suppressor gene. We evaluated the mRNA expression of the FHIT gene in samples from 55 patients with various haematological malignancies (21 AML, 8 CML, 10 CLL, seven low-grade and nine high-grade Non-Hodgkin's lymphomas), in a panel of 16 leukaemia cell lines, in normal mature haematopoietic cells of both myeloid and lymphoid lineage, as well as in CD34+ haematopoietic progenitor cells. Aberrant FHIT mRNA transcripts were observed in 14/16 (88%) leukaemia cell lines, 43/55 (78%) primary haematological neoplasms, but also in 17/22 (77%) normal controls. 1/16 (6%) cell lines and 7/55 (13%) neoplasms did not express any FHIT mRNA. cDNA sequencing revealed exonic deletions, small DNA insertions and combinations of both. Analysis of genomic DNA showed gene deletions in two myeloid leukaemia cell lines. In contrast to all normal types of haematopoietic cells, FHIT protein was clearly reduced or absent in 8/18 (44%) neoplastic samples tested. Our data indicate that whilst aberrant FHIT mRNA transcripts are seen both in normal and malignant cells, lack of FHIT protein is restricted to leukaemia. Absent FHIT protein expression might contribute to leukaemogenesis.

Duke Scholars

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

January 7, 1999

Volume

18

Issue

1

Start / End Page

79 / 85

Location

England

Related Subject Headings

  • U937 Cells
  • Tumor Cells, Cultured
  • RNA, Messenger
  • Proteins
  • Protein Biosynthesis
  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • Leukemia
  • Humans
  • Hematopoietic Stem Cells
 

Citation

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Peters, U. R., Hasse, U., Oppliger, E., Tschan, M., Ong, S. T., Rassool, F. V., … Fey, M. F. (1999). Aberrant FHIT mRNA transcripts are present in malignant and normal haematopoiesis, but absence of FHIT protein is restricted to leukaemia. Oncogene, 18(1), 79–85. https://doi.org/10.1038/sj.onc.1202256
Peters, U. R., U. Hasse, E. Oppliger, M. Tschan, S. T. Ong, F. V. Rassool, B. Borisch, A. Tobler, and M. F. Fey. “Aberrant FHIT mRNA transcripts are present in malignant and normal haematopoiesis, but absence of FHIT protein is restricted to leukaemia.Oncogene 18, no. 1 (January 7, 1999): 79–85. https://doi.org/10.1038/sj.onc.1202256.
Peters UR, Hasse U, Oppliger E, Tschan M, Ong ST, Rassool FV, et al. Aberrant FHIT mRNA transcripts are present in malignant and normal haematopoiesis, but absence of FHIT protein is restricted to leukaemia. Oncogene. 1999 Jan 7;18(1):79–85.
Peters, U. R., et al. “Aberrant FHIT mRNA transcripts are present in malignant and normal haematopoiesis, but absence of FHIT protein is restricted to leukaemia.Oncogene, vol. 18, no. 1, Jan. 1999, pp. 79–85. Pubmed, doi:10.1038/sj.onc.1202256.
Peters UR, Hasse U, Oppliger E, Tschan M, Ong ST, Rassool FV, Borisch B, Tobler A, Fey MF. Aberrant FHIT mRNA transcripts are present in malignant and normal haematopoiesis, but absence of FHIT protein is restricted to leukaemia. Oncogene. 1999 Jan 7;18(1):79–85.

Published In

Oncogene

DOI

ISSN

0950-9232

Publication Date

January 7, 1999

Volume

18

Issue

1

Start / End Page

79 / 85

Location

England

Related Subject Headings

  • U937 Cells
  • Tumor Cells, Cultured
  • RNA, Messenger
  • Proteins
  • Protein Biosynthesis
  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • Leukemia
  • Humans
  • Hematopoietic Stem Cells