Mutation of bcl-x gene in non-Hodgkin's lymphoma.

Journal Article (Journal Article)

The bcl-x gene product has two forms, bcl-xl and bcl-xs. The bcl-xl form, similar to bcl-2, inhibits apoptosis. Reverse transcriptase-polymerase chain reaction/single-stranded conformation polymorphism (RT-PCR-SSCP) gel analysis was used to screen for mutations of the bcl-xl transcript of 50 non-Hodgkin's lymphoma (NHL) cases. One missense mutation in a patient with diffuse large B-cell lymphoma was found. Sequence analysis of this case showed that AGC (Ser) was mutated to GGC (Gly) in codon 154. An examination of mutation and/or polymorphism in born marrow samples of this case and 50 normal controls by the RT-PCR-SSCP method could not detect bandshifts. Mutation of the bcl-x gene in NHL has not been reported previously. There is a possibility that mutation of the bcl-x gene play a role in the tumorigenesis of NHL.

Full Text

Duke Authors

Cited Authors

  • Yamaguchi, H; Inokuchi, K; Tarusawa, M; Dan, K

Published Date

  • January 2002

Published In

Volume / Issue

  • 69 / 1

Start / End Page

  • 74 - 76

PubMed ID

  • 11835337

International Standard Serial Number (ISSN)

  • 0361-8609

Digital Object Identifier (DOI)

  • 10.1002/ajh.10030


  • eng

Conference Location

  • United States