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β2-Glycoprotein I-dependent anticardiolipin antibodies preferentially bind the amino terminal domain of β2-glycoprotein I

Publication ,  Journal Article
McNeeley, PA; Dlott, JS; Furie, RA; Jack, RM; Ortel, TL; Triplett, DA; Victoria, EJ; Linnik, MD
Published in: Thrombosis and Haemostasis
2001

Many of the autoantibodies in antiphospholipid syndrome (APS) are directed against β2-glycoprotein I (β2-GPI). Recent studies from our laboratories have indicated that the immunodominant binding epitope(s) for high titer, affinity purified antibodies from 11 APS patients are localized to the amino terminal domain (domain 1) of β2-GPI. The present study employed surface plasmon resonance to localize the immunodominant domain in serum samples from a large cohort of patients with GPL values ranging from 21 to 230 units (n = 106 patients). Eighty-eight percent of patients showed ≥ threefold selectivity for β2-GPI containing domain 1 relative to the domain deletion mutant that lacked domain 1. The domain 1 binding activity in patient serum was abolished by removing the IgG fraction from the serum and the binding activity could be fully reconstituted with the IgG fraction. Thus, analysis of serum samples from a large cohort of APS patients indicates that the immunodominant binding epitope(s) for anti-β2 antibodies are localized to the amino terminal domain of β2-GPI.

Duke Scholars

Published In

Thrombosis and Haemostasis

Publication Date

2001

Volume

86

Issue

2

Start / End Page

590 / 595

Related Subject Headings

  • Cardiovascular System & Hematology
  • 1103 Clinical Sciences
  • 1102 Cardiorespiratory Medicine and Haematology
 

Citation

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McNeeley, P. A., Dlott, J. S., Furie, R. A., Jack, R. M., Ortel, T. L., Triplett, D. A., … Linnik, M. D. (2001). β2-Glycoprotein I-dependent anticardiolipin antibodies preferentially bind the amino terminal domain of β2-glycoprotein I. Thrombosis and Haemostasis, 86(2), 590–595.
McNeeley, P. A., J. S. Dlott, R. A. Furie, R. M. Jack, T. L. Ortel, D. A. Triplett, E. J. Victoria, and M. D. Linnik. “β2-Glycoprotein I-dependent anticardiolipin antibodies preferentially bind the amino terminal domain of β2-glycoprotein I.” Thrombosis and Haemostasis 86, no. 2 (2001): 590–95.
McNeeley PA, Dlott JS, Furie RA, Jack RM, Ortel TL, Triplett DA, et al. β2-Glycoprotein I-dependent anticardiolipin antibodies preferentially bind the amino terminal domain of β2-glycoprotein I. Thrombosis and Haemostasis. 2001;86(2):590–5.
McNeeley, P. A., et al. “β2-Glycoprotein I-dependent anticardiolipin antibodies preferentially bind the amino terminal domain of β2-glycoprotein I.” Thrombosis and Haemostasis, vol. 86, no. 2, 2001, pp. 590–95.
McNeeley PA, Dlott JS, Furie RA, Jack RM, Ortel TL, Triplett DA, Victoria EJ, Linnik MD. β2-Glycoprotein I-dependent anticardiolipin antibodies preferentially bind the amino terminal domain of β2-glycoprotein I. Thrombosis and Haemostasis. 2001;86(2):590–595.

Published In

Thrombosis and Haemostasis

Publication Date

2001

Volume

86

Issue

2

Start / End Page

590 / 595

Related Subject Headings

  • Cardiovascular System & Hematology
  • 1103 Clinical Sciences
  • 1102 Cardiorespiratory Medicine and Haematology