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Decreased synthesis of matrix metalloproteinase-7 and adhesion to the extracellular matrix proteins of human colon cancer cells treated with troglitazone.

Publication ,  Journal Article
Sunami, E; Tsuno, NH; Kitayama, J; Saito, S; Osada, T; Yamaguchi, H; Tomozawa, S; Tsuruo, T; Shibata, Y; Nagawa, H
Published in: Surg Today
2002

PURPOSE: In the present study, we investigated the effect of troglitazone, a selective ligand and agonist of PPAR-gamma, on the metastatic potential of human colon cancer cells. METHODS: High- and low-PPAR-gamma expression clones of the colon cancer cell line, HT29, namely clones 21 and 3 respectively, were used. We investigated the effect of troglitazone on the proliferation, on the adhesion to extracellular matrix proteins and on the synthesis of matrix metalloproteinases (MMPs) of colon cancer cells. RESULTS: Troglitazone inhibited the proliferation of both subclones, in a dose-dependent manner, and the inhibitory effect correlated with the level of PPAR-gamma expression. Troglitazone strongly inhibited the production of MMP-7, an enzyme associated with invasiveness of cancer cells, by both subclones. In addition, troglitazone caused a strong decrease in the adhesion of clone 21 to extracellular matrix (ECM) proteins, laminin and type IV collagen. This effect was independent of beta1-integrins expression CONCLUSION: In addition to inhibition of cancer cell growth, troglitazone had an inhibitory effect on two important events associated with the metastatic potential of cancer cells, production of MMPs and adhesion to ECM proteins. Consequently, troglitazone is a promising agent for the treatment and prevention of colon cancer metastasis.

Duke Scholars

Published In

Surg Today

DOI

ISSN

0941-1291

Publication Date

2002

Volume

32

Issue

4

Start / End Page

343 / 350

Location

Japan

Related Subject Headings

  • Tumor Cells, Cultured
  • Troglitazone
  • Transcription Factors
  • Thiazolidinediones
  • Thiazoles
  • Surgery
  • Receptors, Cytoplasmic and Nuclear
  • Matrix Metalloproteinase 7
  • Laminin
  • Integrins
 

Citation

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MLA
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Sunami, E., Tsuno, N. H., Kitayama, J., Saito, S., Osada, T., Yamaguchi, H., … Nagawa, H. (2002). Decreased synthesis of matrix metalloproteinase-7 and adhesion to the extracellular matrix proteins of human colon cancer cells treated with troglitazone. Surg Today, 32(4), 343–350. https://doi.org/10.1007/s005950200049
Sunami, Eiji, Nelson Hirokazu Tsuno, Joji Kitayama, Shinsuke Saito, Takuya Osada, Hironori Yamaguchi, Shigeru Tomozawa, Takashi Tsuruo, Yoichi Shibata, and Hirokazu Nagawa. “Decreased synthesis of matrix metalloproteinase-7 and adhesion to the extracellular matrix proteins of human colon cancer cells treated with troglitazone.Surg Today 32, no. 4 (2002): 343–50. https://doi.org/10.1007/s005950200049.
Sunami E, Tsuno NH, Kitayama J, Saito S, Osada T, Yamaguchi H, et al. Decreased synthesis of matrix metalloproteinase-7 and adhesion to the extracellular matrix proteins of human colon cancer cells treated with troglitazone. Surg Today. 2002;32(4):343–50.
Sunami, Eiji, et al. “Decreased synthesis of matrix metalloproteinase-7 and adhesion to the extracellular matrix proteins of human colon cancer cells treated with troglitazone.Surg Today, vol. 32, no. 4, 2002, pp. 343–50. Pubmed, doi:10.1007/s005950200049.
Sunami E, Tsuno NH, Kitayama J, Saito S, Osada T, Yamaguchi H, Tomozawa S, Tsuruo T, Shibata Y, Nagawa H. Decreased synthesis of matrix metalloproteinase-7 and adhesion to the extracellular matrix proteins of human colon cancer cells treated with troglitazone. Surg Today. 2002;32(4):343–350.
Journal cover image

Published In

Surg Today

DOI

ISSN

0941-1291

Publication Date

2002

Volume

32

Issue

4

Start / End Page

343 / 350

Location

Japan

Related Subject Headings

  • Tumor Cells, Cultured
  • Troglitazone
  • Transcription Factors
  • Thiazolidinediones
  • Thiazoles
  • Surgery
  • Receptors, Cytoplasmic and Nuclear
  • Matrix Metalloproteinase 7
  • Laminin
  • Integrins