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E-selectin can mediate the arrest type of adhesion of colon cancer cells under physiological shear flow.

Publication ,  Journal Article
Kitayama, J; Tsuno, N; Sunami, E; Osada, T; Muto, T; Nagawa, H
Published in: Eur J Cancer
January 2000

The aim of this study was to determine whether colon cancer cells flowing in blood exhibit the same adhesion pattern to the vascular bed as leucocytes using a flow adhesion system. In shear flow conditions, five colon cancer cell lines showed less tethering to E-selectin substrates than polymorphonuclear cells (PMN). However, some of the Colo201 cells formed complete arrest on E-selectin in continuous shear flow which was never observed in PMN cells. Colo201 cells expressed both sialyl Le-x and sialyl Le-a at similar levels in flow cytometry. However, the staining pattern showed marked contrast under the fluorescein microscope. The cell membrane of Colo201 cells was uniformly stained with anti-sialyl Le-a MAb, whereas anti-sialyl Le-x MAb only stained in the patchy areas. Pretreatment of Colo201 cells with anti-sLe-a decreased tethering, while anti-sLe-x significantly inhibited the arrest formation. Our data suggest that E-selectin alone can mediate colon cancer cell lodgement and subsequent metastasis without the contribution of integrin molecules and that the different distribution of E-selectin ligands may affect the adhesion behaviour of colon cancer cells in flow conditions.

Duke Scholars

Published In

Eur J Cancer

DOI

ISSN

0959-8049

Publication Date

January 2000

Volume

36

Issue

1

Start / End Page

121 / 127

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Neutrophils
  • Humans
  • Flow Cytometry
  • E-Selectin
  • Colonic Neoplasms
  • Cell Movement
  • Cell Adhesion
  • 3211 Oncology and carcinogenesis
 

Citation

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Kitayama, J., Tsuno, N., Sunami, E., Osada, T., Muto, T., & Nagawa, H. (2000). E-selectin can mediate the arrest type of adhesion of colon cancer cells under physiological shear flow. Eur J Cancer, 36(1), 121–127. https://doi.org/10.1016/s0959-8049(99)00228-2
Kitayama, J., N. Tsuno, E. Sunami, T. Osada, T. Muto, and H. Nagawa. “E-selectin can mediate the arrest type of adhesion of colon cancer cells under physiological shear flow.Eur J Cancer 36, no. 1 (January 2000): 121–27. https://doi.org/10.1016/s0959-8049(99)00228-2.
Kitayama J, Tsuno N, Sunami E, Osada T, Muto T, Nagawa H. E-selectin can mediate the arrest type of adhesion of colon cancer cells under physiological shear flow. Eur J Cancer. 2000 Jan;36(1):121–7.
Kitayama, J., et al. “E-selectin can mediate the arrest type of adhesion of colon cancer cells under physiological shear flow.Eur J Cancer, vol. 36, no. 1, Jan. 2000, pp. 121–27. Pubmed, doi:10.1016/s0959-8049(99)00228-2.
Kitayama J, Tsuno N, Sunami E, Osada T, Muto T, Nagawa H. E-selectin can mediate the arrest type of adhesion of colon cancer cells under physiological shear flow. Eur J Cancer. 2000 Jan;36(1):121–127.
Journal cover image

Published In

Eur J Cancer

DOI

ISSN

0959-8049

Publication Date

January 2000

Volume

36

Issue

1

Start / End Page

121 / 127

Location

England

Related Subject Headings

  • Tumor Cells, Cultured
  • Oncology & Carcinogenesis
  • Neutrophils
  • Humans
  • Flow Cytometry
  • E-Selectin
  • Colonic Neoplasms
  • Cell Movement
  • Cell Adhesion
  • 3211 Oncology and carcinogenesis