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Laminin mediates tethering and spreading of colon cancer cells in physiological shear flow.

Publication ,  Journal Article
Kitayama, J; Nagawa, H; Tsuno, N; Osada, T; Hatano, K; Sunami, E; Saito, H; Muto, T
Published in: Br J Cancer
August 1999

Under the physiological shear condition, cultured colon cancer cells bound to laminin (LM), but not to fibronectin or vitronectin. Most of the tethered cells did not roll, but arrested immediately and spread within 10-30 min on LM under the continuous presence of shear flow. The tethering of Colo201 was partially inhibited by monoclonal antibodies (mAbs) to alpha6 integrin and a combination of mAbs to beta1 and beta4 integrins, but not by mAb to 67KD laminin receptor. Some Colo201 cells still tethered at 4 degrees C. This suggests that alpha6beta1 and alpha6beta4 integrins participate in Colo201 tethering on LM, although other non-integrin molecules play roles. In contrast, the spread of Colo201 was effectively inhibited by the mAbs to integrin alpha2, alpha6 and beta1 chains. The effect of anti-alpha2 plus anti-alpha6 mAbs was almost equal to anti-beta1, suggesting that Colo201 cells mainly use alpha2beta1 and alpha6beta1 integrins for spreading on LM. When the cells were perfused on subconfluent endothelial cells (HUVEC) cultured on LM, they did not tether on HUVEC but did on coated LM exposed at intercellular gap area. Immunohistochemistry revealed that LM abundantly existed in the cytosol of human portal and hepatic vein endothelial cells. These data suggest that LM can mediate from tethering to spreading of colon cancer cells under the blood flow and plays an essential role in haematogeneous metastasis.

Duke Scholars

Published In

Br J Cancer

DOI

ISSN

0007-0920

Publication Date

August 1999

Volume

80

Issue

12

Start / End Page

1927 / 1934

Location

England

Related Subject Headings

  • Vitronectin
  • Umbilical Veins
  • Tumor Cells, Cultured
  • Stress, Mechanical
  • Receptors, Laminin
  • Portal Vein
  • Oncology & Carcinogenesis
  • Models, Biological
  • Laminin
  • Kinetics
 

Citation

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Kitayama, J., Nagawa, H., Tsuno, N., Osada, T., Hatano, K., Sunami, E., … Muto, T. (1999). Laminin mediates tethering and spreading of colon cancer cells in physiological shear flow. Br J Cancer, 80(12), 1927–1934. https://doi.org/10.1038/sj.bjc.6690622
Kitayama, J., H. Nagawa, N. Tsuno, T. Osada, K. Hatano, E. Sunami, H. Saito, and T. Muto. “Laminin mediates tethering and spreading of colon cancer cells in physiological shear flow.Br J Cancer 80, no. 12 (August 1999): 1927–34. https://doi.org/10.1038/sj.bjc.6690622.
Kitayama J, Nagawa H, Tsuno N, Osada T, Hatano K, Sunami E, et al. Laminin mediates tethering and spreading of colon cancer cells in physiological shear flow. Br J Cancer. 1999 Aug;80(12):1927–34.
Kitayama, J., et al. “Laminin mediates tethering and spreading of colon cancer cells in physiological shear flow.Br J Cancer, vol. 80, no. 12, Aug. 1999, pp. 1927–34. Pubmed, doi:10.1038/sj.bjc.6690622.
Kitayama J, Nagawa H, Tsuno N, Osada T, Hatano K, Sunami E, Saito H, Muto T. Laminin mediates tethering and spreading of colon cancer cells in physiological shear flow. Br J Cancer. 1999 Aug;80(12):1927–1934.

Published In

Br J Cancer

DOI

ISSN

0007-0920

Publication Date

August 1999

Volume

80

Issue

12

Start / End Page

1927 / 1934

Location

England

Related Subject Headings

  • Vitronectin
  • Umbilical Veins
  • Tumor Cells, Cultured
  • Stress, Mechanical
  • Receptors, Laminin
  • Portal Vein
  • Oncology & Carcinogenesis
  • Models, Biological
  • Laminin
  • Kinetics