Effect of intracerebroventricular infusion of insulin on glucose-dependent insulinotropic peptide in dogs.
Journal Article (Journal Article)
UNLABELLED: Glucose-dependent insulinotropic polypeptide (GIP), is an incretin with important role in glucose homeostasis and energy conservation. Thus far, the neural/hormonal mechanisms involved in the regulation of GIP secretion, have not yet been fully elucidated. The aim of this study was to evaluate a possible effect of intracerebroventricular administration of insulin in a centrally mediated regulation of GIP. METHODS: Twenty-four adult dogs were used in this study. In group 1 the animals received a bolus icv infusion of regular insulin in a total volume of 50 microl or an equivalent amount of artificial cerebrospinal fluid (aCSF). In group 2 the animals received a continuous icv infusion of insulin or aCSF over a 3-h period. In group 3 the experiment of group 2 was repeated with a simultaneous intraduodenal infusion of a glucose load through the Mann-Bollman fistula. Blood samples were taken from cannulation of a hind limb vein at -15, 0, 5, 10, 15, 30, 45, 60, 90, 120, 150 and 180 min after infusions. Plasma levels of glucose, insulin and GIP were assayed. RESULTS: Insulin levels were increased significantly in group 2 and 3 while GIP secretion was partly inhibited after icv administration of insulin and intraduodenal administration of glucose in the 3rd group. CONCLUSIONS: It is suggested that the hypothalamic insulin signaling contributes to plasma insulin levels and possibly exerts a negative regulation of GIP secretion after glucose load.
Full Text
Duke Authors
Cited Authors
- Yavropoulou, MP; Kotsa, K; Anastasiou, O; O'Dorisio, TM; Pappas, TN; Yovos, JG
Published Date
- August 28, 2009
Published In
Volume / Issue
- 460 / 2
Start / End Page
- 148 - 151
PubMed ID
- 19477229
Electronic International Standard Serial Number (EISSN)
- 1872-7972
Digital Object Identifier (DOI)
- 10.1016/j.neulet.2009.05.052
Language
- eng
Conference Location
- Ireland