SUMO2/3 conjugation is an endogenous neuroprotective mechanism.

Journal Article (Journal Article)

Small ubiquitin-like modifier (SUMO)2/3 but not SUMO1 conjugation is activated after transient cerebral ischemia. To investigate its function, we blocked neuronal SUMO2/3 translation through lentiviral microRNA delivery in primary cortical neurons. Viability was unaffected by SUMO2/3 silencing unless neurons were stressed by transient oxygen-glucose deprivation (OGD). Both 15 and 45 minutes of OGD were tolerated by control microRNA-expressing neurons but damaged >60% of neurons expressing SUMO2/3 microRNA. Damaging OGD (75 minutes) increased neuronal loss to 54% (control microRNA) and to 99% (SUMO2/3 microRNA). This suggests that activation of SUMO2/3 conjugation is an endogenous neuroprotective stress response.

Full Text

Duke Authors

Cited Authors

  • Datwyler, AL; Lättig-Tünnemann, G; Yang, W; Paschen, W; Lee, SLL; Dirnagl, U; Endres, M; Harms, C

Published Date

  • November 2011

Published In

Volume / Issue

  • 31 / 11

Start / End Page

  • 2152 - 2159

PubMed ID

  • 21863037

Pubmed Central ID

  • PMC3210338

Electronic International Standard Serial Number (EISSN)

  • 1559-7016

Digital Object Identifier (DOI)

  • 10.1038/jcbfm.2011.112


  • eng

Conference Location

  • United States