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Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy.

Publication ,  Journal Article
Wang, XQ; Yang, PF; Liu, Z; Liu, WZ; Hu, Y; Chen, H; Kuang, TY; Pei, ZM; Shen, SH; He, YK
Published in: Plant physiology
April 2009

The moss Physcomitrella patens has been shown to tolerate abiotic stresses, including salinity, cold, and desiccation. To better understand this plant's mechanism of desiccation tolerance, we have applied cellular and proteomic analyses. Gametophores were desiccated over 1 month to 10% of their original fresh weight. We report that during the course of dehydration, several related processes are set in motion: plasmolysis, chloroplast remodeling, and microtubule depolymerization. Despite the severe desiccation, the membrane system maintains integrity. Through two-dimensional gel electrophoresis and image analysis, we identified 71 proteins as desiccation responsive. Following identification and functional categorization, we found that a majority of the desiccation-responsive proteins were involved in metabolism, cytoskeleton, defense, and signaling. Degradation of cytoskeletal proteins might result in cytoskeletal disassembly and consequent changes in the cell structure. Late embryogenesis abundant proteins and reactive oxygen species-scavenging enzymes are both prominently induced, and they might help to diminish the damage brought by desiccation.

Duke Scholars

Published In

Plant physiology

DOI

EISSN

1532-2548

ISSN

0032-0889

Publication Date

April 2009

Volume

149

Issue

4

Start / End Page

1739 / 1750

Related Subject Headings

  • Tandem Mass Spectrometry
  • Stress, Physiological
  • Signal Transduction
  • Proteomics
  • Proteome
  • Plant Proteins
  • Plant Biology & Botany
  • Microtubules
  • Electrophoresis, Gel, Two-Dimensional
  • Desiccation
 

Citation

APA
Chicago
ICMJE
MLA
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Wang, X. Q., Yang, P. F., Liu, Z., Liu, W. Z., Hu, Y., Chen, H., … He, Y. K. (2009). Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy. Plant Physiology, 149(4), 1739–1750. https://doi.org/10.1104/pp.108.131714
Wang, Xiao Qin, Ping Fang Yang, Zheng Liu, Wei Zhong Liu, Yong Hu, Hui Chen, Ting Yun Kuang, Zhen Ming Pei, Shi Hua Shen, and Yi Kun He. “Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy.Plant Physiology 149, no. 4 (April 2009): 1739–50. https://doi.org/10.1104/pp.108.131714.
Wang XQ, Yang PF, Liu Z, Liu WZ, Hu Y, Chen H, et al. Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy. Plant physiology. 2009 Apr;149(4):1739–50.
Wang, Xiao Qin, et al. “Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy.Plant Physiology, vol. 149, no. 4, Apr. 2009, pp. 1739–50. Epmc, doi:10.1104/pp.108.131714.
Wang XQ, Yang PF, Liu Z, Liu WZ, Hu Y, Chen H, Kuang TY, Pei ZM, Shen SH, He YK. Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy. Plant physiology. 2009 Apr;149(4):1739–1750.

Published In

Plant physiology

DOI

EISSN

1532-2548

ISSN

0032-0889

Publication Date

April 2009

Volume

149

Issue

4

Start / End Page

1739 / 1750

Related Subject Headings

  • Tandem Mass Spectrometry
  • Stress, Physiological
  • Signal Transduction
  • Proteomics
  • Proteome
  • Plant Proteins
  • Plant Biology & Botany
  • Microtubules
  • Electrophoresis, Gel, Two-Dimensional
  • Desiccation