Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy.

Journal Article (Journal Article)

The moss Physcomitrella patens has been shown to tolerate abiotic stresses, including salinity, cold, and desiccation. To better understand this plant's mechanism of desiccation tolerance, we have applied cellular and proteomic analyses. Gametophores were desiccated over 1 month to 10% of their original fresh weight. We report that during the course of dehydration, several related processes are set in motion: plasmolysis, chloroplast remodeling, and microtubule depolymerization. Despite the severe desiccation, the membrane system maintains integrity. Through two-dimensional gel electrophoresis and image analysis, we identified 71 proteins as desiccation responsive. Following identification and functional categorization, we found that a majority of the desiccation-responsive proteins were involved in metabolism, cytoskeleton, defense, and signaling. Degradation of cytoskeletal proteins might result in cytoskeletal disassembly and consequent changes in the cell structure. Late embryogenesis abundant proteins and reactive oxygen species-scavenging enzymes are both prominently induced, and they might help to diminish the damage brought by desiccation.

Full Text

Duke Authors

Cited Authors

  • Wang, XQ; Yang, PF; Liu, Z; Liu, WZ; Hu, Y; Chen, H; Kuang, TY; Pei, ZM; Shen, SH; He, YK

Published Date

  • April 2009

Published In

Volume / Issue

  • 149 / 4

Start / End Page

  • 1739 - 1750

PubMed ID

  • 19211702

Pubmed Central ID

  • PMC2663739

Electronic International Standard Serial Number (EISSN)

  • 1532-2548

International Standard Serial Number (ISSN)

  • 0032-0889

Digital Object Identifier (DOI)

  • 10.1104/pp.108.131714


  • eng