Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy.
The moss Physcomitrella patens has been shown to tolerate abiotic stresses, including salinity, cold, and desiccation. To better understand this plant's mechanism of desiccation tolerance, we have applied cellular and proteomic analyses. Gametophores were desiccated over 1 month to 10% of their original fresh weight. We report that during the course of dehydration, several related processes are set in motion: plasmolysis, chloroplast remodeling, and microtubule depolymerization. Despite the severe desiccation, the membrane system maintains integrity. Through two-dimensional gel electrophoresis and image analysis, we identified 71 proteins as desiccation responsive. Following identification and functional categorization, we found that a majority of the desiccation-responsive proteins were involved in metabolism, cytoskeleton, defense, and signaling. Degradation of cytoskeletal proteins might result in cytoskeletal disassembly and consequent changes in the cell structure. Late embryogenesis abundant proteins and reactive oxygen species-scavenging enzymes are both prominently induced, and they might help to diminish the damage brought by desiccation.
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Related Subject Headings
- Tandem Mass Spectrometry
- Stress, Physiological
- Signal Transduction
- Proteomics
- Proteome
- Plant Proteins
- Plant Biology & Botany
- Microtubules
- Electrophoresis, Gel, Two-Dimensional
- Desiccation
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Tandem Mass Spectrometry
- Stress, Physiological
- Signal Transduction
- Proteomics
- Proteome
- Plant Proteins
- Plant Biology & Botany
- Microtubules
- Electrophoresis, Gel, Two-Dimensional
- Desiccation