Research at channel level on the effect of LaCl3 on Radish (Raphanus satirus L.) vacuolar membrane

Journal Article (Journal Article)

We recorded slow vacuolar (SV-type) channel currents of Radish vacuoles successfully for the first time by using the whole-vacuolar patch-clamp recording mode. SV-type currents would increase and threshold potentials of activation would shift towards more negative values with the increase of concentrations of cytosolic Ca . When 2.5 mmol/L LaCl and 4 mmol/L EGTA were added to bath solutions, SV-type currents were suppressed remarkably. Then adding LaCl with different concentrations to pipette solutions, we found that LaCl with higher concentrations (> 4 × 10 mol/L) had a strong inhibitory effect on SV-type currents, while LaCl with lower concentrations (≤4 × 10 mol/L) promoted channel currents. This promoting effect provides an important basis at channel level for researching further the effects of rare earth on physiological activities of plants and the production-increase effects of rare earth fertilizers on crops. 2+ -7 -7 3 3 3 3

Duke Authors

Cited Authors

  • Shang, Y; Yang, P; Pei, Z

Published Date

  • January 1, 2002

Published In

Volume / Issue

  • 47 / 2

Start / End Page

  • 112 - 115

International Standard Serial Number (ISSN)

  • 1001-6538

Citation Source

  • Scopus