Action Mechanism Research of Lanthanons to Slow Vacuolar Ion Channels in Raphanus Satirus L. (Xinlimei) Radish by Patch-Clamp

Journal Article (Journal Article)

We used whole-vacuolar patch-clamp recording mode to study the action mechanism of La to Slow Vacuolar (SV) channels for the first time. We recorded SV channel currents of Xinlimei (Raphanus satirus L.) vacuolars. The minimum activation potentials of voltage-dependent SV channels lied in 25±5 mV. The increase in cytoplasmic Ca led to enhancement of SV-type currents. It was found that the threshold potential of activation shifted towards more depolarized values whenever cytoplasmic Ca was increased. When 10 mol/L free La was added to the bath, SV-type current was suppressed by 60-75%. These data showed La reduced ion permeabilities of Xinlimei root vacuolar membrane. 3+ 2+ 2+ -10 3+ 3+

Duke Authors

Cited Authors

  • Yang, P; Shang, YF; Pei, ZM

Published Date

  • September 1, 2001

Published In

Volume / Issue

  • 12 / 9

Start / End Page

  • 813 - 814

International Standard Serial Number (ISSN)

  • 1001-8417

Citation Source

  • Scopus