Cloning the Cryptococcus neoformans TRP1 gene by complementation in Saccharomyces cerevisiae.


Journal Article

We have cloned the phosphoribosyl anthranilate isomerase (PRAI)-encoding gene (TRP1) of Cryptococcus neoformans by genetic complementation in Saccharomyces cerevisiae. Sequence analysis of this gene revealed it to be 939 bp in length, and without known promoter or termination sequences. Unlike some of the filamentous fungi, where PRAI enzymatic activity is controlled by a trifunctional gene product, the C. neoformans PRAI appears to be unifunctional. PRAI of C. neoformans exhibits 39% amino acid (aa) sequence identity compared to the S. cerevisiae counterpart. The TRP1 gene of C. neoformans maps to different size chromosomes in strains with different serotypes. The cloning of this gene for vector constructions, and the demonstration that S. cerevisiae can be used as a surrogate for C. neoformans gene expression, should help with the molecular studies of this significant fungal pathogen in our increasing immunocompromised population.

Full Text

Duke Authors

Cited Authors

  • Perfect, JR; Rude, TH; Penning, LM; Johnson, SA

Published Date

  • December 1, 1992

Published In

Volume / Issue

  • 122 / 1

Start / End Page

  • 213 - 217

PubMed ID

  • 1452032

Pubmed Central ID

  • 1452032

International Standard Serial Number (ISSN)

  • 0378-1119

Digital Object Identifier (DOI)

  • 10.1016/0378-1119(92)90053-r


  • eng

Conference Location

  • Netherlands