Genomic deletions and point mutations induced in Saccharomyces cerevisiae by the trinucleotide repeats (GAA·TTC) associated with Friedreich's ataxia.

Published

Journal Article

Expansion of certain trinucleotide repeats causes several types of human diseases, and such tracts are associated with the formation of deletions and other types of genetic rearrangements in Escherichia coli, yeast, and mammalian cells. Below, we show that long (230 repeats) tracts of the trinucleotide associated with Friedreich's ataxia (GAA·TTC) stimulate both large (>50 bp) deletions and point mutations in a reporter gene located more than 1 kb from the repetitive tract. Sequence analysis of deletion breakpoints indicates that the deletions reflect non-homologous end joining of double-stranded DNA breaks (DSBs) initiated in the tract. The tract-induced point mutations appear to reflect a different mechanism involving single-strand annealing of DNA molecules generated by DSBs within the tract, followed by filling-in of single-stranded gaps by the error-prone DNA polymerase zeta.

Full Text

Duke Authors

Cited Authors

  • Tang, W; Dominska, M; Gawel, M; Greenwell, PW; Petes, TD

Published Date

  • January 1, 2013

Published In

Volume / Issue

  • 12 / 1

Start / End Page

  • 10 - 17

PubMed ID

  • 23182423

Pubmed Central ID

  • 23182423

Electronic International Standard Serial Number (EISSN)

  • 1568-7856

Digital Object Identifier (DOI)

  • 10.1016/j.dnarep.2012.10.001

Language

  • eng

Conference Location

  • Netherlands