Meiotic chromosome segregation in triploid strains of Saccharomyces cerevisiae.

Published

Journal Article

Meiosis in triploids results in four highly aneuploid gametes because six copies of each homolog must be segregated into four meiotic products. Using DNA microarrays and other physical approaches, we examined meiotic chromosome segregation in triploid strains of Saccharomyces cerevisiae. In most tetrads with four viable spores, two of the spores had two copies of a given homolog and two spores had only one copy. Chromosomes segregated randomly into viable spores without preferences for generating near haploid or near diploid spores. Using single-nucleotide polymorphisms, we showed that, in most tetrads, all three pairs of homologs recombined. Strains derived from some of the aneuploid spore colonies had very high frequencies of mitotic chromosome loss, resulting in genetically diverse populations of cells.

Full Text

Duke Authors

Cited Authors

  • St Charles, J; Hamilton, ML; Petes, TD

Published Date

  • October 2010

Published In

Volume / Issue

  • 186 / 2

Start / End Page

  • 537 - 550

PubMed ID

  • 20697121

Pubmed Central ID

  • 20697121

Electronic International Standard Serial Number (EISSN)

  • 1943-2631

Digital Object Identifier (DOI)

  • 10.1534/genetics.110.121533

Language

  • eng

Conference Location

  • United States