Meiotic chromosome segregation in triploid strains of Saccharomyces cerevisiae.
Journal Article (Journal Article)
Meiosis in triploids results in four highly aneuploid gametes because six copies of each homolog must be segregated into four meiotic products. Using DNA microarrays and other physical approaches, we examined meiotic chromosome segregation in triploid strains of Saccharomyces cerevisiae. In most tetrads with four viable spores, two of the spores had two copies of a given homolog and two spores had only one copy. Chromosomes segregated randomly into viable spores without preferences for generating near haploid or near diploid spores. Using single-nucleotide polymorphisms, we showed that, in most tetrads, all three pairs of homologs recombined. Strains derived from some of the aneuploid spore colonies had very high frequencies of mitotic chromosome loss, resulting in genetically diverse populations of cells.
Full Text
Duke Authors
Cited Authors
- St Charles, J; Hamilton, ML; Petes, TD
Published Date
- October 2010
Published In
Volume / Issue
- 186 / 2
Start / End Page
- 537 - 550
PubMed ID
- 20697121
Pubmed Central ID
- PMC2942868
Electronic International Standard Serial Number (EISSN)
- 1943-2631
Digital Object Identifier (DOI)
- 10.1534/genetics.110.121533
Language
- eng
Conference Location
- United States