A fine-structure map of spontaneous mitotic crossovers in the yeast Saccharomyces cerevisiae.

Published

Journal Article

Homologous recombination is an important mechanism for the repair of DNA damage in mitotically dividing cells. Mitotic crossovers between homologues with heterozygous alleles can produce two homozygous daughter cells (loss of heterozygosity), whereas crossovers between repeated genes on non-homologous chromosomes can result in translocations. Using a genetic system that allows selection of daughter cells that contain the reciprocal products of mitotic crossing over, we mapped crossovers and gene conversion events at a resolution of about 4 kb in a 120-kb region of chromosome V of Saccharomyces cerevisiae. The gene conversion tracts associated with mitotic crossovers are much longer (averaging about 12 kb) than the conversion tracts associated with meiotic recombination and are non-randomly distributed along the chromosome. In addition, about 40% of the conversion events have patterns of marker segregation that are most simply explained as reflecting the repair of a chromosome that was broken in G1 of the cell cycle.

Full Text

Duke Authors

Cited Authors

  • Lee, PS; Greenwell, PW; Dominska, M; Gawel, M; Hamilton, M; Petes, TD

Published Date

  • March 2009

Published In

Volume / Issue

  • 5 / 3

Start / End Page

  • e1000410 -

PubMed ID

  • 19282969

Pubmed Central ID

  • 19282969

Electronic International Standard Serial Number (EISSN)

  • 1553-7404

Digital Object Identifier (DOI)

  • 10.1371/journal.pgen.1000410

Language

  • eng

Conference Location

  • United States