High rates of "unselected" aneuploidy and chromosome rearrangements in tel1 mec1 haploid yeast strains.

Published

Journal Article

The yeast TEL1 and MEC1 genes (homologous to the mammalian ATM and ATR genes, respectively) serve partially redundant roles in the detection of DNA damage and in the regulation of telomere length. Haploid yeast tel1 mec1 strains were subcultured nonselectively for approximately 200 cell divisions. The subcultured strains had very high rates of chromosome aberrations: duplications, deletions, and translocations. The breakpoints of the rearranged chromosomes were within retrotransposons (Ty or delta-repeats), and these chromosome aberrations nonrandomly involved chromosome III. In addition, we showed that strains with the hypomorphic mec1-21 allele often became disomic for chromosome VIII. This property of the mec1-21 strains is suppressed by a plasmid containing the DNA2 gene (located on chromosome VIII) that encodes an essential nuclease/helicase involved in DNA replication and DNA repair.

Full Text

Duke Authors

Cited Authors

  • Vernon, M; Lobachev, K; Petes, TD

Published Date

  • May 2008

Published In

Volume / Issue

  • 179 / 1

Start / End Page

  • 237 - 247

PubMed ID

  • 18458104

Pubmed Central ID

  • 18458104

International Standard Serial Number (ISSN)

  • 0016-6731

Digital Object Identifier (DOI)

  • 10.1534/genetics.107.086603

Language

  • eng

Conference Location

  • United States