High rates of "unselected" aneuploidy and chromosome rearrangements in tel1 mec1 haploid yeast strains.
Journal Article (Journal Article)
The yeast TEL1 and MEC1 genes (homologous to the mammalian ATM and ATR genes, respectively) serve partially redundant roles in the detection of DNA damage and in the regulation of telomere length. Haploid yeast tel1 mec1 strains were subcultured nonselectively for approximately 200 cell divisions. The subcultured strains had very high rates of chromosome aberrations: duplications, deletions, and translocations. The breakpoints of the rearranged chromosomes were within retrotransposons (Ty or delta-repeats), and these chromosome aberrations nonrandomly involved chromosome III. In addition, we showed that strains with the hypomorphic mec1-21 allele often became disomic for chromosome VIII. This property of the mec1-21 strains is suppressed by a plasmid containing the DNA2 gene (located on chromosome VIII) that encodes an essential nuclease/helicase involved in DNA replication and DNA repair.
Full Text
Duke Authors
Cited Authors
- Vernon, M; Lobachev, K; Petes, TD
Published Date
- May 2008
Published In
Volume / Issue
- 179 / 1
Start / End Page
- 237 - 247
PubMed ID
- 18458104
Pubmed Central ID
- PMC2390603
International Standard Serial Number (ISSN)
- 0016-6731
Digital Object Identifier (DOI)
- 10.1534/genetics.107.086603
Language
- eng
Conference Location
- United States